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Article
Use of Pi5(t) markers in marker-assisted selection to screen for cultivars with resistance to Magnaporthe grisea
Identification of the PCR markers tightly linked to genes that encode important agronomic traits is useful for marker-assisted selection (MAS). The rice Pi5(t) locus confers broad-spectrum resistance to Magnaport...
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Article
High-resolution genetic map** of Xa27(t), a new bacterial blight resistance gene in rice, Oryza sativa L.
Bacterial blight of rice, caused by Xanthomonas oryzae pv. oryzae (Xoo) (Ishyama) Dye, is one of the serious diseases prevalent throughout Asia. In a previous study, a resistance (R) locus was transferred from th...
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Article
Isolation and characterization of rice mutants compromised in Xa21-mediated resistance to X. oryzae pv. oryzae
The rice gene, Xa21, confers resistance to diverse races of Xanthomonas oryzae pv. oryzae (Xoo) and encodes a receptor-like kinase with leucine-rich repeats in the extra-cellular domain. To identify genes essenti...
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Article
Isolation and characterization of novel defense response genes involved in compatible and incompatible interactions between rice and Magnaporthe grisea
To identify early-induced defense genes involved in broad-spectrum resistance to rice blast, suppression subtractive hybridization was used to generate two cDNA libraries enriched for transcripts differentiall...
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Article
Development of a new transformation-competent artificial chromosome (TAC) vector and construction of tomato and rice TAC libraries
Recent research has shown that BIBAC (binary bacterial artificial chromosome) and TAC (transformation-competent artificial chromosome) vector systems are very useful tools for map-based cloning of agronomicall...
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Article
Evidence of multiple complex patterns of T-DNA integration into the rice genome
The transfer of the long T-DNA (T-DNA and non-T-DNA) of a binary plasmid from Agrobacterium into the rice genome was investigated at both molecular and genetic levels. Out of 226 independent transgenic plants, 33...
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Article
Construction of a bacterial artificial chromosome library containing large Eco RI and Hin dIII genomic fragments of lettuce
Existing bacterial artificial chromosome (BAC) vectors were modified to have unique EcoRI cloning sites. This provided an additional site for generating representative libraries from genomic DNA digested with a ...
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Article
The distribution of Gossypium hirsutum chromatin in G. barbadense germ plasm: molecular analysis of introgressive plant breeding
Cotton is unusual among major crop plants in that two cross-fertile species are widely cultivated for a common economic product, fiber. Both historical evidence and classical genetic studies suggest that many ...
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Article
Assessment of DNA pooling strategies for map** of QTLs
The synthesis of “DNA pools” from segregating populations is an efficient strategy for identifying DNA markers closely linked to genes or genomic regions of interest. To-date, DNA pooling based solely upon phe...