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Article
Mi-2 complex couples DNA methylation to chromatin remodelling and histone deacetylation
Methylation of DNA at the dinucleotide CpG is essential for mammalian development and is correlated with stable transcriptional silencing1,2,3. This transcriptional silencing has recently been linked at a molecul...
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Article
Stage-specific chromosomal association of Drosophila dMBD2/3 during genome activation
The Drosophila gene dMBD2/3 encodes a protein with significant homologies to the mammalian methyl-DNA binding proteins MBD2 and MBD3. These proteins are essential components of chromatin complexes involved in ep...
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Chapter
The Epigenetic Breakdown of Cancer Cells: From DNA Methylation to Histone Modifications
The recognition of epigenetic defects in all types of cancer has represented a revolutionary achievement in cancer research in recent years. DNA methylation aberrant changes (global hypomethylation and CpG isl...
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Article
The impact of MECP2 mutations in the expression patterns of Rett syndrome patients
Rett syndrome (RTT), the second most common cause of mental retardation in females, has been associated with mutations in MeCP2, the archetypical member of the methyl-CpG binding domain (MBD) family of protein...
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Article
Loss of acetylation at Lys16 and trimethylation at Lys20 of histone H4 is a common hallmark of human cancer
CpG island hypermethylation and global genomic hypomethylation are common epigenetic features of cancer cells. Less attention has been focused on histone modifications in cancer cells. We characterized post-tr...
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Article
A truncating mutation of HDAC2 in human cancers confers resistance to histone deacetylase inhibition
Disruption of histone acetylation patterns is a common feature of cancer cells, but very little is known about its genetic basis. We have identified truncating mutations in one of the primary human histone dea...
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Chapter
A Changing Epigenome in Health and Disease
In mammals, epigenetic modifications play an essential role both in establishing transcription profiles and in organizing DNA architecture within the cell nucleus. Specifically, the role of epigenetic modifica...
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Chapter
Examining DNA–Protein Interactions with Genome-Wide Chromatin Immunoprecipitation Analysis
Understanding the mechanisms by which genomic information is hierarchically organized and used by different cell and tissue types under different physiological conditions requires the detailed analysis of the ...
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Article
Epigenetics Lessons from Twins: Prospects for Autoimmune Disease
The existence of phenotypic differences between monozygotic (MZ) twins is a prime case where the relationship between genetic determinants and environmental factors is illustrated. Although virtually identical...
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Chapter
An Introduction to Epigenetics
Eukaryotic genomic information is modulated by a variety of epigenetic modifications that play both a direct role in establishing transcription profiles, modulation of DNA replication and repair processes and ...
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Article
Epigenetic alterations in autoimmune rheumatic diseases
The potential of epigenetics to explain the complex links between environmental triggers and genetic susceptibility is captivating researchers in many diseases. As this article describes, considerable evidence...
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Article
Open AccessEpigenetic alterations in autoimmune disease
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Chapter
Disruption of Epigenetic Mechanisms in Autoimmune Syndromes
Autoimmune disorders are a heterogeneous group of both systemic and organ-specific diseases characterized by the abnormal recognition of self-components by the immune system. These are genetically complex dise...
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Article
Open AccessThe B cell transcription program mediates hypomethylation and overexpression of key genes in Epstein-Barr virus-associated proliferative conversion
Epstein-Barr virus (EBV) infection is a well characterized etiopathogenic factor for a variety of immune-related conditions, including lymphomas, lymphoproliferative disorders and autoimmune diseases. EBV-medi...
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Article
Open AccessPU.1 target genes undergo Tet2-coupled demethylation and DNMT3b-mediated methylation in monocyte-to-osteoclast differentiation
DNA methylation is a key epigenetic mechanism for driving and stabilizing cell-fate decisions. Local deposition and removal of DNA methylation are tightly coupled with transcription factor binding, although th...
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Protocol
Chromatin Immunoprecipitation
Chromatin plays important functions in regulating many biological processes, including DNA transcription, replication, and repair. The use of chromatin immunoprecipitation (ChIP) assays has contributed enormou...
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Article
Geriatric muscle stem cells switch reversible quiescence into senescence
Regeneration of skeletal muscle depends on a population of adult stem cells (satellite cells) that remain quiescent throughout life. Satellite cell regenerative functions decline with ageing. Here we report th...
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Article
Open AccessData integration in the era of omics: current and future challenges
To integrate heterogeneous and large omics data constitutes not only a conceptual challenge but a practical hurdle in the daily analysis of omics data. With the rise of novel omics technologies and through lar...
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Article
Epigenetic control of myeloid cell differentiation, identity and function
The interplay between transcription factors and epigenetic regulators is crucial for regulating gene-expression programmes during haematopoiesis. Epigenetic re...
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Article
Open AccessNF-κB-direct activation of microRNAs with repressive effects on monocyte-specific genes is critical for osteoclast differentiation
Monocyte-to-osteoclast conversion is a unique terminal differentiation process that is exacerbated in rheumatoid arthritis and bone metastasis. The mechanisms implicated in upregulating osteoclast-specific gen...
