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Chapter
Maize Alcohol Dehydrogenase: A Molecular Perspective
During the 1960’s a number of advances were made toward understanding the regulation of prokaryotic and fungal gene systems. From there interest turned to gene regulation in higher eukaryotes and, amongst plan...
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Article
Molecular analysis of a somaclonal mutant of maize alcohol dehydrogenase
Plants regenerated from tissue cultures of maize were screened for variants of ADH1 and ADH2. Root extracts of 645 primary regenerant plants were tested, and one stable mutant of Adh1 was detected. The mutant gen...
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Article
Common evolutionary origin of legume and non-legume plant haemoglobins
The detection of haemoglobins in distantly-related non-legume plant families1,2 as well as in the legume families raises the question of whether the origin of the plant haemoglobins was single or multiple, We hav...
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Article
Functioning haemoglobin genes in non-nodulating plants
Haemoglobin has previously been recorded in plants only in the nitrogen-fixing nodules formed by symbiotic association between Rhizobium or Frankia and legume or non-legume hosts11–4. Structural similarities amon...
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Article
Molecular analysis of the alcohol dehydrogenase gene family of barley
One partial and two complete genomic clones of the three loci specifying alcohol dehydrogenase (ADH) in barley were isolated by screening libraries with a maize Adh1 cDNA probe. Each gene is characterised by an i...
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Article
Transcription of the maize transposable element Ac in maize seedlings and in transgenic tobacco
A combination of cDNA cloning and S1 analyses of RNA isolated from maize seedlings that carry an active Ac element has been used to define the Ac transcript. The primary transcript contains 4 introns that are exc...
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Article
Organ regulated expression of the Parasponia andersonii haemoglobin gene in transgenic tobacco plants
Plant haemoglobin genes are known to occur in legume and non-legume families and in both nodulating (e.g. Parasponia andersonii) and non-nodulating species (e.g. Trema tomentosa). Their presence in non-nodulating...
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Article
The maize transposable element Ac excises in progeny of transformed tobacco
To assess the potential of the maize transposable element Ac for gene tagging in heterologous plant species we monitored transcription, excision and transposition of the element in transgenic tobacco plants and t...
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Article
Molecular analysis of the ADH1-C m allele of maize
The Adh1-C mallele and each gene in the Adh1-FC mduplication have been cloned and restriction-mapped. Of the C mallele 6 kb was sequenced. A single amino acid substitution of aspartate for tyrosine at residue 52 ...
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Article
Factors influencing stable transformation of maize protoplasts by electroporation
Two chimaeric genes, containing the promoter of the 35S gene of cauliflower mosaic virus coupled to neomycin phosphotransferase (35S-NPT-NOS) or to hygromycin phosphotransferase (35S-HPT-NOS) have been stably ...
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Article
Expression of a bacterial gene in transgenic tobacco plants confers resistance to the herbicide 2,4-dichlorophenoxyacetic acid
Plants resistant to the herbicide 2,4-dichlorophenoxyacetic acid (2,4-D) were produced through the genetic engineering of a novel detoxification pathway into the cells of a species normally sensitive to 2,4-D....
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Article
Functional properties of the anaerobic responsive element of the maize Adh1 gene
The functional properties of the anaerobic responsive element (ARE) of the maize Adh1 gene have been analysed using a transient expression assay in electroporated maize protoplasts. The ARE functions in both orie...
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Chapter
The Anaerobic Responsive Element
The response to anaerobic stress such as occurs during flooding, is one of the best-characterised stress responses of plants, at the physiological and biochemical levels. Recent advances in molecular biology h...
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Article
Agrobacterium tumefaciens-gene transfer into wheat tissues
DNA can be transferred by Agrobacterium tumefaciens to wheat, albeit at very low frequencies. Transfer of agrobacterial DNA occurred in cultures where the embryos had been subjected to partial enzymatic digestion...
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Article
Reactivation of a silent Ac following tissue culture is associated with heritable alterations in its methylation pattern
Tissue cultures were initiated from embryos with an inactive form of Ac in the wx-m9 Ds-cy allele. Plants regenerated from the cultures showed a high frequency of activation of Ac. That activation was shown to be...
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Article
Cotton plants transformed with a bacterial degradation gene are protected from accidental spray drift damage by the herbicide 2,4-dichlorophenoxyacetic acid
The agronomic performance of broad leaved crop plants such as cotton would be greatly improved if genetically-engineered resistance to broadleaf herbicides could both protect the plants from accidental spray d...
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Article
Behaviour of modified Ac elements in flax callus and regenerated plants
The Ac element of maize has been modified by deletion of 537 bases (ΔNaeAc) from the untranslated leader of the transposase gene. In a second modification the cauliflower mosaic virus 35S promoter has been insert...
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Chapter
Genetic and molecular dissection of male-fertility in higher plants
Angiosperms use three systems of reproduction: hermaphroditism, in which the flowers are bisexual and predominantly self-pollinating; monoecy, in which the flowers are unisexual, with male and female flowers o...
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Article
Modification of Vacuolar Chitinase and Osmotin cDNAs of Tobacco for Extracellular Secretion and their Cloning with CaMV 35S Promoter
Complete coding region clones of basic chitinase and osmotin genes were obtained using PCR from cDNAs previously isolated from tobacco floral bud day 7 (Fb7) explants. These genes code for vacuolar forms of ch...
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Article
Isolation and characterisation of full-length cDNA clones of the giant taro (Alocasia macrorrhiza) trypsin/chymotrypsin inhibitor
A full-length cDNA encoding the 206 amino acid open reading frame of a trypsin/chymotrypsin inhibitor abundant in the corms of giant taro (Alocasia macrorrhiza) was isolated. An internal fragment was cloned using...
