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Article
Molecular analysis of the alcohol dehydrogenase gene family of barley
One partial and two complete genomic clones of the three loci specifying alcohol dehydrogenase (ADH) in barley were isolated by screening libraries with a maize Adh1 cDNA probe. Each gene is characterised by an i...
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Article
The maize transposable element Ac excises in progeny of transformed tobacco
To assess the potential of the maize transposable element Ac for gene tagging in heterologous plant species we monitored transcription, excision and transposition of the element in transgenic tobacco plants and t...
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Article
Molecular analysis of the ADH1-C m allele of maize
The Adh1-C mallele and each gene in the Adh1-FC mduplication have been cloned and restriction-mapped. Of the C mallele 6 kb was sequenced. A single amino acid substitution of aspartate for tyrosine at residue 52 ...
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Article
Factors influencing stable transformation of maize protoplasts by electroporation
Two chimaeric genes, containing the promoter of the 35S gene of cauliflower mosaic virus coupled to neomycin phosphotransferase (35S-NPT-NOS) or to hygromycin phosphotransferase (35S-HPT-NOS) have been stably ...
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Article
Expression of a bacterial gene in transgenic tobacco plants confers resistance to the herbicide 2,4-dichlorophenoxyacetic acid
Plants resistant to the herbicide 2,4-dichlorophenoxyacetic acid (2,4-D) were produced through the genetic engineering of a novel detoxification pathway into the cells of a species normally sensitive to 2,4-D....
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Article
Functional properties of the anaerobic responsive element of the maize Adh1 gene
The functional properties of the anaerobic responsive element (ARE) of the maize Adh1 gene have been analysed using a transient expression assay in electroporated maize protoplasts. The ARE functions in both orie...
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Article
Agrobacterium tumefaciens-gene transfer into wheat tissues
DNA can be transferred by Agrobacterium tumefaciens to wheat, albeit at very low frequencies. Transfer of agrobacterial DNA occurred in cultures where the embryos had been subjected to partial enzymatic digestion...
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Article
Behaviour of modified Ac elements in flax callus and regenerated plants
The Ac element of maize has been modified by deletion of 537 bases (ΔNaeAc) from the untranslated leader of the transposase gene. In a second modification the cauliflower mosaic virus 35S promoter has been insert...
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Article
Isolation and characterisation of full-length cDNA clones of the giant taro (Alocasia macrorrhiza) trypsin/chymotrypsin inhibitor
A full-length cDNA encoding the 206 amino acid open reading frame of a trypsin/chymotrypsin inhibitor abundant in the corms of giant taro (Alocasia macrorrhiza) was isolated. An internal fragment was cloned using...
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Article
The alcohol dehydrogenase genes of cotton
We report here the initial characterization of the alcohol dehydrogenase (Adh) gene family of the allotetraploid Gossypium hirsutum, a crop plant that is highly sensitive to waterlogging. Twelve Adh cDNAs were is...
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Article
Characterization of pyruvate decarboxylase genes from rice
The pdc1 gene encoding pyruvate decarboxylase has been isolated and sequenced from an IR54 rice genomic library. In contrast to a previously isolated intron-less rice genomic pdc, pRgpdc3, this gene contains five...
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Article
Adaptation of Helicoverpa armigera (Lepidoptera: Noctuidae) to a proteinase inhibitor expressed in transgenic tobacco
A giant taro proteinase inhibitor (GTPI) cDNA was expressed in transgenic tobacco using three different gene constructs. The highest expression level obtained was ca. 0.3% of total soluble protein when the cDN...
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Article
Eucalyptus has a functional equivalent of the Arabidopsis floral meristem identity gene LEAFY
Two genes cloned from Eucalyptus globulus, Eucalyptus LeaFy (ELF1 and ELF2), have sequence homology to the floral meristem identity genes LEAFY from Arabidopsis and FLORICAULA from Antirrhinum. ELF1 is express...
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Article
Multiple DNA methyltransferase genes in Arabidopsis thaliana
Methylation of plant DNA occurs at cytosines in any sequence context, and as the Arabidopsis methyltransferase, METI, preferentially methylates cytosines in CG dinucleotides, it is likely that Arabidopsis has ...
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Article
Expression of the Talaromyces flavus glucose oxidase gene in cotton and tobacco reduces fungal infection, but is also phytotoxic
Glucose oxidase secreted by the fungus Talaromyces flavus generates, in the presence of glucose, hydrogen peroxide that is toxic to phytopathogenic fungi responsible for economically important diseases in many...
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Article
Differential induction of chitinase activity in flax (Linum usitatissimum) in response to inoculation with virulent or avirulent strains of Melampsora lini, the cause of flax rust
Chitinase enzymes are induced in many plants in response to pathogen challenge and other stress stimuli. Chitinase induction in flax leaves in response to inoculation with flax rust, caused by Melampsora lini (Pe...
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Article
Site specificity of the Arabidopsis METI DNA methyltransferase demonstrated through hypermethylation of the superman locus
Plants with low levels of DNA methylation show a range of developmental abnormalities including homeotic transformation of floral organs. Two independent DNA METHYLTRANSFERASEI (METI) antisense transformants with...
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Article
Genome wide gene expression in artificially synthesized amphidiploids of Arabidopsis
The merging of two different genomes occurs during the formation of amphidiploids, and the merged regulatory networks have the potential to generate a new gene expression pattern. We examined the genome-wide g...
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Article
Identification of candidate genes for fusarium yellows resistance in Chinese cabbage by differential expression analysis
Fusarium yellows caused by Fusarium oxysporum f. sp. conglutinans is an important disease of Brassica worldwide. To identify a resistance (R) gene against Fusarium yellows in Chinese cabbage (Brassica rapa var. p...
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Article
Open AccessCharacterization of FLOWERING LOCUS C 5 in Brassica rapa L.
Brassica rapa L., which includes Chinese cabbage, turnip, and pak choi, has more complex flowering time regulation than does Arabidopsis thaliana due to the presence of multiple paralogous flowering time genes.