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Article
Epidemiology, pathogenesis, immune evasion mechanism and vaccine development of porcine Deltacoronavirus
Coronaviruses have been identified as pathogens of gastrointestinal and respiratory diseases in humans and various animal species. In recent years, the global spread of new coronaviruses has had profound influ...
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Article
Porcine endogenous retrovirus: classification, molecular structure, regulation, function, and potential risk in xenotransplantation
Xenotransplantation with porcine organs has been recognized as a promising solution to alleviate the shortage of organs for human transplantation. Porcine endogenous retrovirus (PERV), whose proviral DNAs are ...
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Article
Validation of reliable safe harbor locus for efficient porcine transgenesis
Transgenic technology is now widely used in biomedical and agricultural fields. Transgenesis is commonly achieved through random integration which might cause some uncertain consequences. The site-specific int...
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Article
Construction and application of the vectors to identify genes encoding exported proteins of Escherichia coli
In order to clone genes having signal sequences of Escherichia coli, four vectors with or without Lac or Ara promoter were constructed using a leaderless β-lactamase as reporter. Fragments of tetracycline resista...
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Article
Expression pattern of L-FABP gene in different tissues and its regulation of fat metabolism-related genes in duck
Liver fatty acid binding protein (L-FABP) is a member of intracellular lipid-binding proteins responsible for the transportation of fatty acids. The expression pattern of duck L-FABP mRNA was examined in this ...
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Article
Effect of immunization with a recombinant cholera toxin B subunit/somatostatin fusion protein on immune response and growth hormone levels in mice
Somatostatin (SS) is a hormone that inhibits growth hormone secretion. Cholera toxin B subunit (CTB) is a widely used adjuvant to improve the immunogenicity of co-administrated antigen. To block the growth hor...
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Article
Differential transcriptome profiles of heads from foragers: comparison between Apis mellifera ligustica and Apis cerana cerana
Given the differences in ecology and biology between Apis cerana cerana and Apis mellifera ligustica, we first used the Illumina–Solexa deep sequencing technology to describe the differences in the heads of A. ce...
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Article
Effect of active immunization against a recombinant mouse granulocyte-macrophage colony-stimulating factor/somatostatin fusion protein on the growth of mice
Female BALB/c mice were actively immunized subcutaneously with a recombinant protein of granulocyte-macrophage colony-stimulating factor (GM-CSF) fused with somatostatin (SS) (GM-CSF/SS). Fifty-four days after...
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Article
A novel SNP of liver-type fatty acid-binding protein gene in duck and its associations with the intramuscular fat
Liver-type fatty acid-binding protein (L-FABP) is a member of intracellular lipid-binding proteins involved in the transportation of fatty acids. We detected the polymorphisms of duck L-FABP gene and its assoc...
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Article
Effect of the C-terminal domains and terminal residues of catalytic domain on enzymatic activity and thermostability of lichenase from Clostridium thermocellum
To elucidate the effects of C-terminal domains of LicMB (mature lichenase from Clostridium thermocellum) and terminal residues of LicMB-CD (catalytic domain of LicMB) on the properties of lichenase, a series of t...
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Article
Secretory expression of K88 (F4) fimbrial adhesin FaeG by recombinant Lactococcus lactis for oral vaccination and its protective immune response in mice