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Article
Open AccessPerturbation of cytokinin and ethylene-signalling pathways explain the strong rooting phenotype exhibited by Arabidopsis expressing the Schizosaccharomyces pombe mitotic inducer, cdc25
Entry into mitosis is regulated by cyclin dependent kinases that in turn are phosphoregulated. In most eukaryotes, phosphoregulation is through WEE1 kinase and CDC25 phosphatase. In higher plants a homologous ...
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Article
The E2FD/DEL2 factor is a component of a regulatory network controlling cell proliferation and development in Arabidopsis
An emerging view of plant cell cycle regulators, including the E2F transcription factors, implicates them in the integration of cell proliferation and development. Arabidopsis encodes six E2F proteins that can...
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Article
Characterization of a gene highly expressed in the Brassica napus pistil that encodes a novel proline-rich protein
Pis 30, a gene highly expressed in Brassica napus pistils and encoding a novel proline-rich protein was isolated and characterized. Sequences homologous to the Brassica Pis 30 gene were found only in Arabidopsis ...
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Article
Characterization of genes expressed during the development ofBrassica napus pollen
The study of the formation of pollen in plants has been the focus of extensive morphologic and cytologic observations. This complex developmental process requires the coordinated activity of both gametophytic ...
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Chapter and Conference Paper
Gene Expression during Brassica napus Pollen Development
The development of pollen is a complex process that requires the coordinate participation of various tissue types and specific gene expression patterns. Both gametophytic (pollen) and sporophytic (anther, tape...
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Article
A gene showing sequence similarity to pectin esterase is specifically expressed in develo** pollen of Brassica napus. Sequences in its 5′ flanking region are conserved in other pollen-specific promoters
Differential screening of a Brassica napus genomic library led to the isolation of the clone named Bp 19 containing a gene which is highly expressed during microspore development. The accumulation of Bp 19 mRNA s...
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Article
Characterization of a pollen-specific gene family from Brassica napus which is activated during early microspore development
In this paper we describe the isolation and characterization of a genomic clone (Bp4) from Brassica napus which contains three members of a pollen-specific multigene family. This family is composed of 10 to 15 cl...
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Article
Dihydrofolate reductase from Daucus carota cell suspension cultures: purification, molecular and kinetic characterization
The purification of dihydrofolate reductase (5, 6, 7, 8 tetrahydrofolate: NADP+ oxidoreductase, E.C.: 1.5.1.3) from Daucus carota to apparent homogeneity, is described. The enzyme is a soluble protein with a mole...