-
Article
Open AccessPanpipes: a pipeline for multiomic single-cell and spatial transcriptomic data analysis
Single-cell multiomic analysis of the epigenome, transcriptome, and proteome allows for comprehensive characterization of the molecular circuitry that underpins cell identity and state. However, the holistic i...
-
Article
Open AccessHigh-resolution transcriptional landscape of xeno-free human induced pluripotent stem cell-derived cerebellar organoids
Current protocols for producing cerebellar neurons from human pluripotent stem cells (hPSCs) often rely on animal co-culture and mostly exist as monolayers, limiting their capability to recapitulate the comple...
-
Article
Open AccessPhenotypic manifestation of α-synuclein strains derived from Parkinson’s disease and multiple system atrophy in human dopaminergic neurons
α-Synuclein is critical in the pathogenesis of Parkinson’s disease and related disorders, yet it remains unclear how its aggregation causes degeneration of human dopaminergic neurons. In this study, we induced...
-
Article
Open AccessTREM2 Alzheimer’s variant R47H causes similar transcriptional dysregulation to knockout, yet only subtle functional phenotypes in human iPSC-derived macrophages
TREM2 is a microglial cell surface receptor, with risk mutations linked to Alzheimer’s disease (AD), including R47H. TREM2 signalling via SYK aids phagocytosis, chemotaxis, survival, and changes to microglial act...
-
Article
Open AccessA single-cell atlas of the human substantia nigra reveals cell-specific pathways associated with neurological disorders
We describe a human single-nuclei transcriptomic atlas for the substantia nigra (SN), generated by sequencing approximately 17,000 nuclei from matched cortical and SN samples. We show that the common genetic r...
-
Article
Open AccessMYC functions are specific in biological subtypes of breast cancer and confers resistance to endocrine therapy in luminal tumours
MYC is amplified in approximately 15% of breast cancers (BCs) and is associated with poor outcome. c-MYC protein is multi-faceted and participates in many aspects of cellular function and is linked with therapeut...
