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    Cell-free expression, purification, and membrane reconstitution for NMR studies of the nonstructural protein 4B from hepatitis C virus

    We describe the expression of the hepatitis C virus nonstructural protein 4B (NS4B), which is an integral membrane protein, in a wheat germ cell-free system, the subsequent purification and characterization of...

    Marie-Laure Fogeron, Vlastimil Jirasko, Susanne Penzel in Journal of Biomolecular NMR (2016)