74 Result(s)
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English
Biomedicine
Biological and Medical Physics, Biophysics
Physical Chemistry
Da**g **a
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Chapter
Functional Sub-states by High-pressure Macromolecular Crystallography
At the molecular level, high-pressure perturbation is of particular interest for biological studies as it allows trap** conformational substates. Moreover, within the context of high-pressure adaptation of d...
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Early Days of Pressure Denaturation Studies of Proteins
The denaturation of protein by pressure has been generally well known since the findings of the perfect coagulation of egg white by a pressure of 7,000 atm within 30 min by Bridgman (J Biol Chem 19:511–512, 19...
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Exploring the Protein Folding Pathway with High-Pressure NMR: Steady-State and Kinetics Studies
Defining the physical-chemical determinants of protein folding and stability, under normal and pathological conditions has constituted a major subfield in biophysical chemistry for over 50 years. Although a gr...
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Driving Forces in Pressure-Induced Protein Transitions
The molecular mechanisms underlying pressure-induced protein denaturation can be analyzed based on the pressure-dependent differences in the apparent volume occupied by amino acids inside the protein and when ...
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Pressure-Inactivated Virus: A Promising Alternative for Vaccine Production
In recent years, many applications in diverse scientific fields with various purposes have examined pressure as a thermodynamic parameter. Pressure studies on viruses have direct biotechnological applications....
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Volume and Compressibility of Proteins
The partial specific (or molar) volume, expansibility, and compressibility of a protein are fundamental thermodynamic quantities for characterizing its structure in solution. We review the definitions, measure...
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Pressure Effects on Artificial and Cellular Membranes
We review the combined effect of temperature and pressure on the structure, dynamics and phase behavior of lipid bilayers, differing in chain length, headgroup structure and composition as revealed by thermody...
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Water Turns the “Non-biological” Fluctuation of Protein into “Biological” One
Structural fluctuation of protein is not just an mechanical “oscillation,” but an event induced by an interplay of mechanical and thermodynamic processes in which water plays crucial role. The chapter is devoted ...
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Environmental Adaptation of Dihydrofolate Reductase from Deep-Sea Bacteria
In order to elucidate the molecular adaptation mechanisms of enzymes to the high hydrostatic pressure of the deep sea, we cloned, purified, and characterized more than ten dihydrofolate reductases (DHFRs) from...
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Homeoviscous Adaptation of Membranes in Archaea
Because membranes play a central role in regulating fluxes inward and outward from the cells, maintaining the appropriate structure of the membrane is crucial to maintain cellular integrity and functions. Micr...
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High Pressure NMR Methods for Characterizing Functional Substates of Proteins
Proteins usually exist in multiple conformational states in solution. High pressure NMR spectroscopy is a well-suited method to identify these states. In addition, these states can be characterized by their th...
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Pressure-Based Strategy for the Inactivation of Spores
Since the first application of high hydrostatic pressure (HHP) for food preservation more than 100 years ago, a wealth of knowledge has been gained on molecular mechanisms underlying the HHP-mediated destructi...
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Use of Pressure for Improving Storage Quality of Fresh-Cut Produce
The microflora of fresh-cut produce is comprised primarily of phytopathogenic and soilborne organisms, but the product could be contaminated with foodborne pathogens. Populations of bacteria, molds, and yeasts...
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High-Pressure Microscopy for Studying Molecular Motors
Movement is a fundamental characteristic of all living things. This biogenic function is carried out by various nanometer-sized molecular machines. Molecular motor is a typical molecular machinery in which the...
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High Pressure Macromolecular Crystallography
X-ray crystallography is a powerful tool for the high resolution structural study of biomacromolecules. One of the most important beneficial feature of the method is the possibility of the direct observation o...
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Gravitational Effects on Human Physiology
Physical working capacity decreases with age and also in microgravity. Regardless of age, increased physical activity can always improve the physical adaptability of the body, although the mechanisms of this a...
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High Pressure NMR Spectroscopy
The combination of high-resolution NMR spectroscopy with pressure perturbation, known as variable-pressure NMR spectroscopy or simply high pressure NMR spectroscopy, is a relatively recent accomplishment, but ...
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High-Pressure NMR Spectroscopy Reveals Functional Sub-states of Ubiquitin and Ubiquitin-Like Proteins
High-pressure nuclear magnetic resonance (NMR) spectroscopy has revealed that ubiquitin has at least two high-energy states – an alternatively folded state N2 and a locally disordered state I – between the basic ...
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Cavities and Excited States in Proteins
Protein cavities or voids are observed as defects in atomic packing. Cavities have long been suggested to play important roles in protein dynamics and function, but the underlying origin and mechanism remains ...
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Protein Denaturation on p-T Axes – Thermodynamics and Analysis
Proteins are essential players in the vast majority of molecular level life processes. Since their structure is in most cases substantial for their correct function, study of their structural changes attracted...
