674 Result(s)
within
Da** Chen
English
Biomedical Engineering/Biotechnology
Biotechnology
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Article
Open AccessPlate fixation of inferior ramus in pubis-ischium ramus improves mechanical stability in Tile B pelvic injures: a cadaveric biomechanical analysis and early clinical experience
Management of inferior ramus of the pubis-ischium ramus remains controversial, and related research is sparse. The main intention of this study is to describe the biomechanical and clinical outcomes of pubis-i...
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Author Correction: High-yield porphyrin production through metabolic engineering and biocatalysis
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In vivo AAV–SB-CRISPR screens of tumor-infiltrating primary NK cells identify genetic checkpoints of CAR-NK therapy
Natural killer (NK) cells have clinical potential against cancer; however, multiple limitations hinder the success of NK cell therapy. Here, we performed unbiased functional map** of tumor-infiltrating NK (T...
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Open AccessNoninvasive prediction of lymph node metastasis in pancreatic cancer using an ultrasound-based clinicoradiomics machine learning model
This study was designed to explore and validate the value of different machine learning models based on ultrasound image-omics features in the preoperative diagnosis of lymph node metastasis in pancreatic canc...
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Open AccessAn in-vitro three-dimensional surgical simulation technique to predict tibial tunnel length in transtibial posterior cruciate ligament reconstruction
During the transtibial posterior cruciate ligament (PCL) reconstruction, drilling depth excessively longer than the tibial tunnel length (TTL) is an important reason to cause popliteal neurovascular bundle inj...
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High-yield porphyrin production through metabolic engineering and biocatalysis
Porphyrins and their derivatives find extensive applications in medicine, food, energy and materials. In this study, we produced porphyrin compounds by combining Rhodobacter sphaeroides as an efficient cell facto...
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Author Correction: Re-engineering the adenine deaminase TadA-8e for efficient and specific CRISPR-based cytosine base editing
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Author Correction: Adenine transversion editors enable precise, efficient A•T-to-C•G base editing in mammalian cells and embryos
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Open AccessAutomatic detection of epilepsy from EEGs using a temporal convolutional network with a self-attention layer
Over 60% of epilepsy patients globally are children, whose early diagnosis and treatment are critical for their development and can substantially reduce the disease’s burden on both families and society. Numer...
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Trans-nuclease activity of Cas9 activated by DNA or RNA target binding
Type V and type VI CRISPR–Cas systems have been shown to cleave nonspecific single-stranded DNA (ssDNA) or single-stranded RNA (ssRNA) in trans, but this has not been observed in type II CRISPR–Cas systems using ...
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Open AccessAuthor Correction: Combined small-molecule treatment accelerates maturation of human pluripotent stem cell-derived neurons
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Open AccessEngineered CRISPR-Cas12a for higher-order combinatorial chromatin perturbations
Multiplexed genetic perturbations are critical for testing functional interactions among coding or non-coding genetic elements. Compared to double-stranded DNA cutting, repressive chromatin formation using CRI...
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Open AccessGeneration of allogeneic CAR-NKT cells from hematopoietic stem and progenitor cells using a clinically guided culture method
Cancer immunotherapy with autologous chimeric antigen receptor (CAR) T cells faces challenges in manufacturing and patient selection that could be avoided by using ‘off-the-shelf’ products, such as allogeneic ...
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Open AccessTracking single-cell evolution using clock-like chromatin accessibility loci
Single-cell chromatin accessibility sequencing (scATAC-seq) reconstructs developmental trajectory by phenotypic similarity. However, inferring the exact developmental trajectory is challenging. Previous studie...
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Open AccessSingle-nucleotide variant calling in single-cell sequencing data with Monopogen
Single-cell omics technologies enable molecular characterization of diverse cell types and states, but how the resulting transcriptional and epigenetic profiles depend on the cell’s genetic background remains ...
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Open Access3D printing of Ceffe-infused scaffolds for tailored nipple-like cartilage development
The reconstruction of a stable, nipple-shaped cartilage graft that precisely matches the natural nipple in shape and size on the contralateral side is a clinical challenge. While 3D printing technology can eff...
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Therapeutic application of circular RNA aptamers in a mouse model of psoriasis
Efforts to advance RNA aptamers as a new therapeutic modality have been limited by their susceptibility to degradation and immunogenicity. In a previous study, we demonstrated synthesized short double-stranded...
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Discovery of tumor-reactive T cell receptors by massively parallel library synthesis and screening
T cell receptor (TCR) gene therapy is a potent form of cellular immunotherapy in which patient T cells are genetically engineered to express TCRs with defined tumor reactivity. However, the isolation of therap...
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Open AccessDevelopment and validation of an ultrasound-based deep learning radiomics nomogram for predicting the malignant risk of ovarian tumours
The timely identification and management of ovarian cancer are critical determinants of patient prognosis. In this study, we developed and validated a deep learning radiomics nomogram (DLR_Nomogram) based on u...
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Author Correction: Tumor immunotherapy across MHC barriers using allogeneic T-cell precursors
