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792 Result(s)

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  1. No Access

    Article

    High efficiency reporter gene transfection of vascular tissue in vitro and in vivo using a cationic lipid–DNA complex

    Efficient transfection conditions for a number of human, rat and rabbit primary cells and established lines of vascular origin have been determined using a complex of a commercially available cationic lipid tr...

    M-C Keogh, D Chen, F Lupu, N Shaper, JF Schmitt, VV Kakkar, NR Lemoine in Gene Therapy (1997)

  2. No Access

    Article

    Improved efficiency of arterial gene transfer by use of poloxamer 407 as a vehicle for adenoviral vectors

    Improvement in the efficiency of adenovirus-mediated arterial gene transfer may augment the utility of cardiovascular gene therapy. In vitro studies suggest that poloxamer 407 enhances transfection efficiency ...

    LJ Feldman, CJ Pastore, N Aubailly, M Kearney, D Chen, M Perricaudet… in Gene Therapy (1997)

  3. No Access

    Article

    Neurotoxicity of intracerebral injection of a replication-defective adenoviral vector in a semipermissive species (cotton rat)

    The neurotoxicity of an adenoviral vector (Adv.RSVtk) carrying the gene for herpes simplex virus thymidine kinase (HSVtk) was tested in the cotton rat, a semipermissive host. Adv.RSVtk was injected intracerebr...

    HD Shine, PR Wyde, E Aguilar-Cordova, S-H Chen, SLC Woo, RG Grossman… in Gene Therapy (1997)

  4. No Access

    Article

    Novel retroviral packaging cell lines: complementary tropisms and improved vector production for efficient gene transfer

    We report increased transduction of human hematopoietic progenitor cells through a combination of novel retroviral vector packaging cell lines, and improved vector supernatant production. The new ProPak packag...

    SP Forestell, JS Dando, J Chen, P de Vries, E Böhnlein, RJ Rigg in Gene Therapy (1997)

  5. No Access

    Article

    Induction of antigen-specific antitumor immunity with adenovirus-transduced dendritic cells

    Transduction of dendritic cells (DC) can result in presentation of tumor-associated antigens and induction of immunity against undefined epitopes. The present studies demonstrate adenovirus (Ad)-mediated trans...

    J Gong, L Chen, D Chen, M Kashiwaba, Y Manome, T Tanaka, D Kufe in Gene Therapy (1997)

  6. No Access

    Article

    A GFP reporter system to assess gene transfer and expression in human hematopoietic progenitor cells

    Hematopoietic stem cells are widely recognized as attractive targets for gene therapy but current protocols to transduce these cells using recombinant retroviral vectors are inefficient. To evaluate optimizati...

    L Cheng, C Du, D Murray, X Tong, YA Zhang, BP Chen, RG Hawley in Gene Therapy (1997)

  7. No Access

    Article

    Gene transfer and expression in oligodendrocytes under the control of myelin basic protein transcriptional control region mediated by adeno-associated virus

    In this study, a rAAV vector carrying a reporter gene, ‘humanized’ green fluorescent protein (GFP), linked to the transcriptional control region from the myelin basic protein (MBP) gene (a myelin-forming cell-...

    H Chen, DM McCarty, AT Bruce, K Suzuki, K Suzuki in Gene Therapy (1998)

  8. No Access

    Article

    Genetic co-inactivation of macrophage- and T-tropic HIV-1 chemokine coreceptors CCR-5 and CXCR-4 by intrakines

    CC-chemokine receptor (CCR)-5 is the principal coreceptor for the entry of macrophage (M)-tropic HIV-1 viruses into a cell, while CXC-chemokine receptor (CXCR)-4 is the principal coreceptor for T cell line (T)...

    X Bai, J-D Chen, A-G Yang, F Torti, S-Y Chen in Gene Therapy (1998)

  9. No Access

    Article

    The effect of synthetic surfactant Exosurf on gene transfer in mouse lung in vivo

    Gene transfer in the lung holds promise for the treatment of diseases such as pulmonary fibrosis, cystic fibrosis and asthma. Pulmonary surfactant has been reported to enhance expression from endobronchial, ad...

    E Raczka, JF Kukowska-Latallo, M Rymaszewski, C Chen, JR Baker Jr in Gene Therapy (1998)

  10. No Access

    Article

    Safety study and characterization of E1A–liposome complex gene-delivery protocol in an ovarian cancer model

    A phase I clinical trial of E1A–liposome complex is currently ongoing in patients with HER-2/neu-overexpressing breast or ovarian cancers. To optimize the E1A–liposome complex for a further stage of clinical t...

    X **ng, S Zhang, JY Chang, SD Tucker, H Chen, L Huang, MC Hung in Gene Therapy (1998)

  11. No Access

    Article

    Treatment of lysosomal storage disease in MPS VII mice using a recombinant adeno-associated virus

    Mucopolysaccharidosis type VII (MPS VII) is a lysosomal storage disease caused by a genetic deficiency of β-glucuronidase (GUS). We used a recombinant adeno-associated virus vector (AAV-GUS) to deliver GUS cDN...

    GL Watson, JN Sayles, C Chen, SS Elliger, CA Elliger, NR Raju, GJ Kurtzman… in Gene Therapy (1998)

  12. No Access

    Article

    Design of a muscle cell-specific expression vector utilising human vascular smooth muscle α-actin regulatory elements

    The facility to direct tissue-specific expression of therapeutic gene constructs is desirable for many gene therapy applications. We describe the creation of a muscle-selective expression vector which supports...

    M-C Keogh, D Chen, J F Schmitt, U Dennehy, V V Kakkar, N R Lemoine in Gene Therapy (1999)

  13. No Access

    Article

    Suppression of HIV-1 transcription and replication by a Vpr mutant

    Vpr, the 96 amino acid long protein represents one of the auxiliary proteins of human immunodeficiency virus type-1 (HIV-1), which exhibits the ability to increase the rate of replication of the virus in T cel...

    B E Sawaya, K Khalili, J Rappaport, D Serio, W Chen, A Srinivasan, S Amini in Gene Therapy (1999)

  14. No Access

    Article

    Tissue-selective expression of dominant-negative proteins for the regulation of vascular smooth muscle cell proliferation

    The transcription factors c-myb and c-myc are essential for vascular smooth muscle cell (VSMC) replication and are rapidly induced following mitogenic stimulation of quiescent VSMCs in vitro and in vivo follow...

    J F Schmitt, M-C Keogh, U Dennehy, D Chen, F Lupu, K Weston, D Taylor… in Gene Therapy (1999)

  15. No Access

    Article

    Gene gun-mediated DNA vaccination induces antitumor immunity against human papillomavirus type 16 E7-expressing murine tumor metastases in the liver and lungs

    DNA vaccination has emerged as an attractive approach for tumor immunotherapy. The aim of this study was to evaluate the potency of DNA vaccines in preventing and treating the liver and lung metastases of a hu...

    C-H Chen, H Ji, K W Suh, M A Choti, D M Pardoll, T-C Wu in Gene Therapy (1999)

  16. No Access

    Article

    Long-term phenotypic correction of rodent hemiparkinsonism by gene therapy using genetically modified myoblasts

    Rat myoblasts were genetically modified to express tyrosine hydroxylase (TH) and produce dopamine in culture. Implanting TH gene-transfected myoblasts into the denervated striatum of 6-OHDA-lesioned rats signi...

    L Cao, Y-C Zhao, Z-H Jiang, D-H Xu, Z-G Liu, S-D Chen, X-Y Liu, Z-C Zheng in Gene Therapy (2000)

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    Article

    Induction of potent antitumor response by vaccination with tumor lysate-pulsed macrophages engineered to secrete macrophage colony-stimulating factor and interferon-γ

    Adoptive transfer of activated macrophages, being both effector cells and antigen-presenting cells, represents a promising approach to immunotherapy of cancer. In order to get activated macrophages with increa...

    H Lei, D W Ju, Y Yu, Q Tao, G Chen, S Gu, H Hamada, X Cao in Gene Therapy (2000)

  18. No Access

    Article

    Effective repeat administration with adenovirus vectors to the muscle

    Effective repeat administration of adenovirus vectors following intranasal or intravenous delivery is hindered by a strong neutralizing antibody response to the vector. Intramuscular administration of adenovir...

    P Chen, I Kovesdi, J T Bruder in Gene Therapy (2000)

  19. Article

    Genetic polymorphism of RhD-negative associated haplotypes in the Chinese

    The Rh blood group is the most polymorphic human blood group system, and is clinically significant in transfusion medicine. Individuals are classified as Rh-positive and Rh-negative depending on the presence ...

    J. Lan, Q. Chen, D. Wu, H. Ding, D. Pong, T. Zhao in Journal of Human Genetics (2000)

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  20. No Access

    Article

    Co-polymer of histidine and lysine markedly enhances transfection efficiency of liposomes

    Development of nonviral delivery systems is progressing toward a transfection efficiency sufficient to affect metabolic and neoplastic diseases in humans. Nevertheless, inadequate transfection efficiency of ta...

    Q-R Chen, L Zhang, S A Stass, A J Mixson in Gene Therapy (2000)

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