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Article
Genetic transformation of tropical maize (Zea mays L.) inbred line with a phytase gene from Aspergillus niger
A full-length cDNA of phyA gene of Aspergillus niger, encoding phytase enzyme, was cloned and expressed in E. coli BL21 cells and assayed for its activity. The phyA cDNA consisted of 1404 bp, which encoded 467 am...
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Engineering Disease Resistance in Rice
Rice diseases cause substantial yield loss in rice. Through conventional breeding, resistance genes (R-gene) were transferred into elite rice genotypes particularly against the fungal blast and bacterial bligh...
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Article
Open AccessTransgenic rice plants expressing synthetic cry2AX1 gene exhibits resistance to rice leaffolder (Cnaphalocrosis medinalis)
Bacillus thuringiensis is a major source of insecticidal genes imparting insect resistance in transgenic plants. Level of expression of transgenes in transgenic plants is important to ach...
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Article
Transgenic rice as a system to study the stability of transgene expression: multiple heterologous transgenes show similar behaviour in diverse genetic backgrounds
The success of contemporary breeding programmes involving genetic engineering depends on the stability of transgene expression over many generations. We studied the stability of transgene expression in 40 inde...