11 Result(s)
within
D. Sudhakar
Life Sciences
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Article
Enhancing rice grain quality through the knock-out of the OsSPL16 gene
The OsSPL16 (SQUAMOSA promoter-binding protein like 16) or GW8 (Grain Width 8) gene is expressed specifically in develo** seeds and positively controls grain width and weight by modulating cell proliferation an...
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Article
Genome editing of indica rice ASD16 for imparting resistance against rice tungro disease
Rice tungro disease (RTD) is caused by the joint infection of rice tungro bacilliform virus and rice tungro spherical virus (RTSV) and is the most important viral disease that limits rice production and produc...
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Chapter
Engineering Disease Resistance in Rice
Rice diseases cause substantial yield loss in rice. Through conventional breeding, resistance genes (R-gene) were transferred into elite rice genotypes particularly against the fungal blast and bacterial bligh...
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Article
Stability of sheath blight resistance in transgenic ASD16 rice lines expressing a rice chi11 gene encoding chitinase
Development of transgenic plants by introducing defense genes is one of the strategies to engineer disease resistance. Transgenic ASD16 rice plants harbouring rice chitinase chi11 gene, belonging to a PR-3 group ...
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Article
Development of leaffolder resistant transgenic rice expressing cry2AX1 gene driven by green tissue-specific rbcS promoter
The insecticidal cry genes of Bacillus thuringiensis (Bt) have been successfully used for development of insect resistant transgenic rice plants. In this study, a novel cry2AX1 gene consisting a sequence of cry2A...
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Article
Expression and inheritance of chimeric cry2AX1 gene in transgenic cotton plants generated through somatic embryogenesis
Transgenic cotton plants expressing a novel cry2AX1 gene consisting of sequences from the cry2Aa and cry2Ac genes, driven by the CaMV35S or the EnCaMV35S promoters, were generated through Agrobacterium-mediated t...
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Article
A maize α-zein promoter drives an endosperm-specific expression of transgene in rice
An alpha-zein promoter isolated from maize containing P-box, E motif sequence TGTAAAGT, opaque-2 box and TATA box was studied for its tissue-specific expression in rice. A 1,098 bp promoter region of alpha-zein g...
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Article
Transcriptome analysis of salinity responsiveness in contrasting genotypes of finger millet (Eleusine coracana L.) through RNA-sequencing
Finger millet (Eleusine coracana L.) is a hardy cereal known for its superior level of tolerance against drought, salinity, diseases and its nutritional properties. In this study, attempts were made to unravel t...
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Article
Pyramiding transgenic resistance in elite indica rice cultivars against the sheath blight and bacterial blight
Elite indica rice cultivars were cotransformed with genes expressing a rice chitinase (chi11) and a thaumatin-like protein (tlp) conferring resistance to fungal pathogens and a serine-threonine kinase (Xa21) conf...
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Article
An improvedAgrobacterium-mediated transformation protocol for recalcitrant elite indica rice cultivars
We report here a high-efficiency transformation protocol for recalcitrant indica rice cultivars IR64 and IR72 with the selectable marker genehph and thegusA reporter gene. Factors that favor high-efficiency trans...
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Article
Transgenic rice as a system to study the stability of transgene expression: multiple heterologous transgenes show similar behaviour in diverse genetic backgrounds
The success of contemporary breeding programmes involving genetic engineering depends on the stability of transgene expression over many generations. We studied the stability of transgene expression in 40 inde...
