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Article
Coordinatively unsaturated cobalt single-atom nanozymes for visual pesticides detection by smartphone-based platform
By adjusting the coordination environment of single-atom catalysts, the enzyme-like activity can be finely tuned for highly sensitive biosensing. Herein, we demonstrated that coordinatively unsaturated cobalt-...
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Article
Atomically-precise Au24Ag1 Clusterzymes with Enhanced Peroxidase-like Activity for Bioanalysis
Atomically-precise clusterzymes have been widely studied for their special physicochemical properties, but it is still a challenge to enhance their peroxidase-like activity. Herein, we demonstrated that by subs.....
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Article
Platinum nanoparticles confined in metal–organic frameworks as excellent peroxidase-like nanozymes for detection of uric acid
High levels of uric acid (UA) in humans can cause a range of diseases, and traditional assays that rely on uric acid enzymes to break down uric acid are limited by the inherent deficiencies of natural enzymes....
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Article
Single-atom Pd catalysts as oxidase mimics with maximum atom utilization for colorimetric analysis
Pd-based nanomaterials have shown great promise as potential mimic enzymes, but conventional catalysts use only a small fraction of the Pd content that located on the catalyst’s surface. Herein, we demonstrate...
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Article
Colorimetric detection of acetylcholinesterase and its inhibitor based on thiol-regulated oxidase-like activity of 2D palladium square nanoplates on reduced graphene oxide
A convenient and sensitive colorimetric assay for acetylcholinesterase (AChE) and its inhibitor has been designed based on the oxidase-like activity of {100}-faceted Pd square nanoplates which are grown in sit...
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Article
Ultrathin PdCu alloy nanosheet–assembled 3D nanoflowers with high peroxidase-like activity toward colorimetric glucose detection
Enzyme-mimetic properties of nanomaterials can be efficiently tuned by controlling their size, composition, and structure. Here, ultrathin PdCu alloy nanosheet–assembled three-dimensional (3D) nanoflowers (Pd1Cux
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Article
Colorimetric determination of the activity of alkaline phosphatase by exploiting the oxidase-like activity of palladium cube@CeO2 core-shell nanoparticles
Core-shell palladium cube@CeO2 (Pd cube@CeO2) nanoparticles are shown to display oxidase-like activity. This is exploited in a method for determination of the activity of alkaline phosphatase (ALP). The Pd cube@C...
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Article
Fluorometric and colorimetric dual-readout alkaline phosphatase activity assay based on enzymatically induced formation of colored Au@Ag nanoparticles and an inner filter effect
An ultrasensitive fluorometric and colorimetric dual-mode assay is described for the determination of the activity of alkaline phosphatase (ALP). ALP catalyzes the decomposition of 2-phospho-L-ascorbic acid, a...
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Article
Spectrophotometric determination of the activity of alkaline phosphatase and detection of its inhibitors by exploiting the pyrophosphate-accelerated oxidase-like activity of nanoceria
The oxidase-like activity of nanoceria is low. This limits its practical applications. It is demonstrated here that pyrophosphate ion (PPi) can improve the oxidase-like activity of nanoceria. Specifically, nan...
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Article
Fluorometric determination of the activity of alkaline phosphatase and its inhibitors based on ascorbic acid-induced aggregation of carbon dots
The authors describe a fluorometric method for determination of the activity of alkaline phosphatase (ALP) and its inhibitors. Nitrogen and boron co-doped carbon dots (C-dots) with excitation/emission peaks at...
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Article
Fluorometric determination of sulfide ions via its inhibitory effect on the oxidation of thiamine by Cu(II) ions
A fluorometric assay is described for sulfide ions determination. It is based on the finding that the oxidation of the non-fluorescent substrate thiamine (TH) by Cu(II) in basic solution to form fluorescent th...