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8 Result(s)

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  1. Article

    Open Access

    Exploring cellular memory molecules marking competent and active transcriptions

    Development in higher eukaryotes involves programmed gene expression. Cell type-specific gene expression is established during this process and is inherited in succeeding cell cycles. Higher eukaryotes have ev...

    Li **n, Guo-Ling Zhou, Wei Song, Xue-Song Wu, Gong-Hong Wei… in BMC Molecular Biology (2007)

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  2. No Access

    Article

    A general method to modify BACs to generate large recombinant DNA fragments

    Bacterial artificial chromosome (BAC) has the capacity to clone DNA fragments in excess of 300 kb. It also has the considerable advantages of stable propagation and ease of purification. These features make BA...

    Wei Shen, Yue Huang, Yi Tang, De-Pei Liu, Chih-Chuan Liang in Molecular Biotechnology (2005)

  3. No Access

    Article

    Knockdown of mouse adult β-globin gene expression in MEL cells by retrovirus vector-mediated RNA interference

    RNA interference (RNAi) efficiently induces sequence-specific gene silencing in mammalian cells through short interfering RNA (siRNA) of 21–23 nucleotides synthesized in vitro or expressed by DNA-based vector....

    Na Zhao, Zhen-**ang Zu, Chang-Mei Liu, Wen-Ji Dong, De-Pei Liu… in Molecular Biotechnology (2004)

  4. No Access

    Article

    Evaluation of optimal expression cassette in retrovirus vector for β-thalassemia gene therapy

    Trials of retroviral vector-mediated human β-globin gene transfer were hampered by low titers, unstable vector transmission, and low-level expression of transferred gene. With the goal of optimizing the retrov...

    Wen-Ji Dong, Bin Li, De-Pei Liu, Zhen-**ang Zu, Jia Li… in Molecular Biotechnology (2003)

  5. No Access

    Article

    Hot topics in adeno-associated virus as a gene transfer vector

    Adeno-associated virus (AAV) is a promising viral vector in treating many kinds of hereditary diseases. The broad host range, low level of immune response, and longevity of gene expression observed with this v...

    Professor Na Zhao, De-Pei Liu, Chih-Chuan Liang in Molecular Biotechnology (2001)

  6. No Access

    Article

    A fast and efficient method for isolation of the BAC end

    As a new developmental vector system, the bacterial artificial chromosome (BAC) has been used widely in constructing genomic libraries and in generating transgenic animals. Isolation of the BAC insert end is u...

    Yue Huang, De-Pei Liu, Min Wu, Chih-Chuan Liang in Molecular Biotechnology (2001)

  7. No Access

    Article

    End-labeling of long DNA fragments with biotin and detection of DNA immobilized on magnetic beads

    To immobilize DNA fragments onto magnetic beads coated with streptavidin for isolation purpose, it is important to label one biotin molecule at one terminus of DNA fragment. After failure to label long DNA wit...

    Haiming Xu, Shen Zhang, Depei Liu, Chih-chuan Liang in Molecular Biotechnology (2001)

  8. No Access

    Article

    Inversion and Transposition of Tc1 Transposon of C. elegans in Mammalian Cells

    Tc1/mariner transposons are widespread in the eukaryotes. In vitro transposition test indicated that the transposase is the only protein that is needed in transpositions. It was shown later that ...

    Zhu-Hong Li, De-Pei Liu, **g Wang, Zhi-Chen Guo… in Somatic Cell and Molecular Genetics (1998)