20 Result(s)
within
Chih-Chuan Liang
English
-
Article
Modulations of hMOF autoacetylation by SIRT1 regulate hMOF recruitment and activities on the chromatin
A wide variety of nuclear regulators and enzymes are subjected to acetylation of the lysine residue, which regulates different aspects of protein functions. The MYST family histone acetyltransferase, human ort...
-
Article
Unravelling the world of cis-regulatory elements
Genome-wide comparisons indicate that only studying the coding regions will not be enough for explaining the biological complexity of an organism, while the genetic variants and the epigenetic differences of cis-...
-
Article
Open AccessExploring cellular memory molecules marking competent and active transcriptions
Development in higher eukaryotes involves programmed gene expression. Cell type-specific gene expression is established during this process and is inherited in succeeding cell cycles. Higher eukaryotes have ev...
-
Article
A20 attenuates vascular smooth muscle cell proliferation and migration through blocking PI3k/Akt singling in vitro and in vivo
A20 was originally characterized as a TNF-inducible gene in human umbilical vein endothelial cells. It is also induced in many other cell types by a wide range of stimuli. Expression of A20 has been shown to p...
-
Article
A general method to modify BACs to generate large recombinant DNA fragments
Bacterial artificial chromosome (BAC) has the capacity to clone DNA fragments in excess of 300 kb. It also has the considerable advantages of stable propagation and ease of purification. These features make BA...
-
Article
Chromatin domain boundaries: insulators and beyond
The eukaryotic genome is organized into functionally and structurally distinct domains, representing regulatory units for gene expression and chromosome behavior. DNA sequences that mark the border between adj...
-
Article
Knockdown of mouse adult β-globin gene expression in MEL cells by retrovirus vector-mediated RNA interference
RNA interference (RNAi) efficiently induces sequence-specific gene silencing in mammalian cells through short interfering RNA (siRNA) of 21–23 nucleotides synthesized in vitro or expressed by DNA-based vector....
-
Article
Paraoxonase gene polymorphisms, oxidative stress, and diseases
The paraoxonase (PON) gene cluster contains at least three members, including PON1, PON2, and PON3, located on chromosome 7q21.3–22.1. Until now there has been little insight into the role of the respective ge...
-
Article
Evaluation of optimal expression cassette in retrovirus vector for β-thalassemia gene therapy
Trials of retroviral vector-mediated human β-globin gene transfer were hampered by low titers, unstable vector transmission, and low-level expression of transferred gene. With the goal of optimizing the retrov...
-
Article
Oligonucleotide-mediated gene repair at DNA level: the potential applications for gene therapy
Mutations in gene sequence can cause many genetic disorders, and researchers have attempted to develop treatments or cures at the DNA level for these diseases. Several strategies including triple-helix-formin...
-
Article
Analysis of adeno-associated virus-mediated ex vivo transferred human β-globin gene in bone marrow engrafted mice
Adeno-associated virus (AAV)-2 was developed as a useful vector for human gene therapy. In this report, we analyzed the integration and expression of AAV-mediated ex vivo transferred human β-globin gene in bon...
-
Article
Hot topics in adeno-associated virus as a gene transfer vector
Adeno-associated virus (AAV) is a promising viral vector in treating many kinds of hereditary diseases. The broad host range, low level of immune response, and longevity of gene expression observed with this v...
-
Article
Insulator: from chromatin domain boundary to gene regulation
An insulator or boundary element is a novel class of regulatory sequence and has properties consistent with a role in limiting enhancer activity; it helps to define the boundary between differentially regulat...
-
Article
Prospects of chimeric RNA-DNA oligonucleotides in gene therapy
A strategy called targeted gene repair was developed to facilitate the process of gene therapy using a chimeric RNA-DNA oligonucleotide. Experiments demonstrated the feasibility of using the chimeric oligonucl...
-
Article
A fast and efficient method for isolation of the BAC end
As a new developmental vector system, the bacterial artificial chromosome (BAC) has been used widely in constructing genomic libraries and in generating transgenic animals. Isolation of the BAC insert end is u...
-
Article
The regulatory network controlling β-globin gene switching
The human globin gene cluster, which represents a prototypical eukaryotic multigene locus, has been investigated for more than two decades and is classic model for coordinate control of tissue-specific gene ex...
-
Article
End-labeling of long DNA fragments with biotin and detection of DNA immobilized on magnetic beads
To immobilize DNA fragments onto magnetic beads coated with streptavidin for isolation purpose, it is important to label one biotin molecule at one terminus of DNA fragment. After failure to label long DNA wit...
-
Article
Inversion and Transposition of Tc1 Transposon of C. elegans in Mammalian Cells
Tc1/mariner transposons are widespread in the eukaryotes. In vitro transposition test indicated that the transposase is the only protein that is needed in transpositions. It was shown later that ...
-
Article
PI LBEI and PI JHOU: two new alpha-1-antitrypsin alleles
Two new variants of alpha-1-antitrypsin were found in a Chinese population by isoelectric focusing in polyacrylamide gel. They have been named Lbei**g (PI*LBEI) and Jhouyao (PI*JHOU) respectively. With the sampl...
-
Article
Types and subtypes of haptoglobin in the Chinese population
Haptoglobin phenotypes of 1121 unrelated Chinese blood donors in Bei**g were determined. The gene frequency of Hp1 was 0.270. A rare variant, which we identified as Hp1S-J, was found. Two hundred and two samples...
