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Chapter
Chemical Modification of a Membrane Protein by Hydrophobic and Hydrophilic Labeling Reagents
The major transmembrane protein of the erythrocyte membrane — band 3 — has been chosen as an example of an integral membrane protein. This polypeptide is known to be involved in anion transport and has therefo...
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Article
Charge clusters and the orientation of membrane proteins
Although hydrophobic forces probably dominate in determining whether or not a protein will insert into a membrane, recent studies in our laboratory suggest that electrostatic forces may influence the final ori...
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Article
Long-term cultivation of Semliki Forest virus (SFV) infectedAedes albopictus cells at pH6
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Article
Involvement of cysteine in Semliki Forest virus (SFV) induced fusion ofAedes albopictus cells
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Article
Alteration of cellular functions after Semliki Forest virus induced fusion at low pH ofAedes albopictus cells
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Article
Semliki Forest virus-induced polykaryocyte formation is an ATP-dependent event
Infection of Aedes albopictus cells with Semliki Forest virus (SFV) leads to polykaryocyte formation below pH 6.2. This syncytium formation is accompanied by a decrease of the cellular ATP level. Addition of i...
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Article
A novel method for the detection of early events in cell-cell fusion of Semliki Forest virus infected cells growing in monolayer cultures
Semliki Forest virus infected Aedes albopictus cells were used to investigate virus induced cell-cell fusion. It was shown by a novel method that cell-cell fusion was completed within approximately 5 minutes a...
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Article
Can viral envelope proteins act as or induce proton channels?
The mechanism of the process leading to cell-cell fusion induced by enveloped viruses at a mildly acidic pH is as yet unknown. In this report we demonstrate that the fusion events induced by three viruses of d...
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Article
Exposure of Semliki Forest virus-infected baby hamster kidney cells to low pH leads to a proton influx and a rapid depletion of intracellular ATP which in turn prevents cell-cell fusion
The conditions needed to trigger Semliki Forest virus (SFV) induced cell-cell fusion from within (FFWI) of baby hamster kidney (BHK) cells differs from some other cells (e.g., Aedes albopictus cells). In contr...
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Article
Dynamic changes in plasma membrane properties of semliki forest virus infected cells related to cell fusion
The mechanism of the processes leading to membrane fusion is as yet unknown. In this report we demonstrate that changes in membrane potential and potassium fluxes correlate with Semliki Forest virus induced ce...
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Article
A comparative study of the effect of dextran sulfate on the fusion and the in vitro replication of influenza A and B, Semliki Forest, vesicular stomatitis, rabies, Sendai, and mumps virus
The effect of dextran sulfate on the fusion of a series of enveloped viruses, bearing specifically different fusion proteins, was investigated. The fusion with model- and with biological membranes was monitore...
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Article
The fragmentation of incoming Semliki Forest virus nucleocapsids in mosquito (Aedes Albopictus) cells might be coupled to virion uncoating
The fate of Semliki Forest virus (SFV) nucleocapsid, especially the capsid protein (C-protein), was investigated during the early stages of a productive infection in mosquitoAedes albopictus cells. Infection of t...
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Article
Cleavage of incoming Semliki Forest virus capsid protein within the endocytotic pathway: a feature common to both invertebrate and mammalian cells
During the initial stages of Semliki Forest virus (SFV) infection in mammalian (baby hamster kidney, BHK) cells, the cleavage of SFV capsid protein could be detected. Analysis of subcellular fractions from SFV...
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Chapter
Herstellung, Verträglichkeit und Virussicherheit von intravenösem Immunglobulin
Die mehr als hundertjährige Geschichte der klinischen Anwendung von Immunglobulinen fangt mit der antitoxischen Wirkung von tierischen Immunseren an. In der Entwicklung hin zu einem nebenwirkungsarmen und biol...
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Article
Combined Calcium and Vitamin D3 Supplementation in Elderly Women: Confirmation of Reversal of Secondary Hyperparathyroidism and Hip Fracture Risk: The Decalyos II Study
Vitamin D insufficiency and low calcium intake contribute to increase parathyroid function and bone fragility in elderly people. Calcium and vitamin D supplements can reverse secondary hyperparathyroidism thus...
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Article
Lenograstim after autologous peripheral blood progenitor cell transplantation: results of a double-blind, randomized trial
A phase III, randomized, double-blind, placebo-controlled, multi-center trial was conducted in order to compare the incidence of microbiologically defined infections occurring after high-dose chemotherapy (HDT...