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  1. Article

    Semliki Forest virus-induced polykaryocyte formation is an ATP-dependent event

    Infection of Aedes albopictus cells with Semliki Forest virus (SFV) leads to polykaryocyte formation below pH 6.2. This syncytium formation is accompanied by a decrease of the cellular ATP level. Addition of i...

    C. Kempf, Ursula Kohler, M. R. Michel, H. Koblet in Archives of Virology (1987)

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  2. Article

    A novel method for the detection of early events in cell-cell fusion of Semliki Forest virus infected cells growing in monolayer cultures

    Semliki Forest virus infected Aedes albopictus cells were used to investigate virus induced cell-cell fusion. It was shown by a novel method that cell-cell fusion was completed within approximately 5 minutes a...

    C. Kempf, M. R. Michel, U. Kohler, H. Koblet in Archives of Virology (1987)

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    Article

    Can viral envelope proteins act as or induce proton channels?

    The mechanism of the process leading to cell-cell fusion induced by enveloped viruses at a mildly acidic pH is as yet unknown. In this report we demonstrate that the fusion events induced by three viruses of d...

    C. Kempf, M. R. Michel, U. Kohler, H. Koblet in Bioscience Reports (1987)

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    Article

    Exposure of Semliki Forest virus-infected baby hamster kidney cells to low pH leads to a proton influx and a rapid depletion of intracellular ATP which in turn prevents cell-cell fusion

    The conditions needed to trigger Semliki Forest virus (SFV) induced cell-cell fusion from within (FFWI) of baby hamster kidney (BHK) cells differs from some other cells (e.g., Aedes albopictus cells). In contr...

    C. Kempf, M. R. Michel, U. Kohler, H. Koblet in Archives of Virology (1988)

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    Article

    Dynamic changes in plasma membrane properties of semliki forest virus infected cells related to cell fusion

    The mechanism of the processes leading to membrane fusion is as yet unknown. In this report we demonstrate that changes in membrane potential and potassium fluxes correlate with Semliki Forest virus induced ce...

    C. Kempf, M. R. Michel, U. Kohler, H. Koblet, H. Oetliker in Bioscience Reports (1988)

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    Article

    A comparative study of the effect of dextran sulfate on the fusion and the in vitro replication of influenza A and B, Semliki Forest, vesicular stomatitis, rabies, Sendai, and mumps virus

    The effect of dextran sulfate on the fusion of a series of enveloped viruses, bearing specifically different fusion proteins, was investigated. The fusion with model- and with biological membranes was monitore...

    M. Lüscher-Mattli, R. Glück, C. Kempf, M. Zanoni-Grassi in Archives of Virology (1993)

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    Article

    The fragmentation of incoming Semliki Forest virus nucleocapsids in mosquito (Aedes Albopictus) cells might be coupled to virion uncoating

    The fate of Semliki Forest virus (SFV) nucleocapsid, especially the capsid protein (C-protein), was investigated during the early stages of a productive infection in mosquitoAedes albopictus cells. Infection of t...

    B. Mrkic, C. Kempf in Archives of Virology (1996)

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    Article

    Cleavage of incoming Semliki Forest virus capsid protein within the endocytotic pathway: a feature common to both invertebrate and mammalian cells

    During the initial stages of Semliki Forest virus (SFV) infection in mammalian (baby hamster kidney, BHK) cells, the cleavage of SFV capsid protein could be detected. Analysis of subcellular fractions from SFV...

    B. Mrkic, T. Tetaz, C. Kempf in Archives of Virology (1997)