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    Protocol

    Retinal Tissue

    Mutations within genes that are specifically expressed within the retina and/or the retinal pigment epithelium (RPE) are associated with the majority of inherited retinal degenerations (1). Retinal degenerative d...

    Trevor McFarland, Binoy Appukuttan in Lentivirus Gene Engineering Protocols (2003)

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    Protocol

    Corneal Cells

    A mammalian cornea can be maintained in vitro for days (1,2), allowing ex vivo transduction of this tissue prior to transplantation (3,4). Ex vivo transduction of the cornea is useful for studying the efficacy of...

    Binoy Appukuttan, Trevor McFarland in Lentivirus Gene Engineering Protocols (2003)