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  1. No Access

    Article

    An Improved Method of Gene Synthesis Based on DNA Works Software and Overlap Extension PCR

    A bottleneck in recent gene synthesis technologies is the high cost of oligonucleotide synthesis and post-synthesis sequencing. In this article, a simple and rapid method for low-cost gene synthesis technology...

    Bingxue Dong, Runqian Mao, Baojian Li, Qiuyun Liu, Peilin Xu in Molecular Biotechnology (2007)

  2. Article

    Open Access

    A novel esterase gene cloned from a metagenomic library from neritic sediments of the South China Sea

    Marine microbes are a large and diverse group, which are exposed to a wide variety of pressure, temperature, salinity, nutrient availability and other environmental conditions. They provide a huge potential so...

    Qing Peng, Xue Zhang, Meng Shang, Xu Wang, Guili Wang in Microbial Cell Factories (2011)

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    Article

    Enzymatic Synthesis of Lipophilic Caffeoyl Lipids Using Soybean Oil as the Novel Acceptor

    Soybean oil-based caffeoyl lipids are the novel lipophilic derivatives of caffeic acid, which can be used as UV absorbers and antioxidants in the food and cosmetic industries. In the work, the novel lipophilic...

    Shangde Sun, Liya Tian, Bingxue Hu, Cong Jiang in Biotechnology and Bioprocess Engineering (2018)

  4. No Access

    Article

    Heparin stimulates biofilm formation of Escherichia coli strain Nissle 1917

    Escherichia coli strain Nissle 1917 (EcN), a gut probiotic competing with pathogenic bacteria, has been used to attenuate various intestinal dysfunctions. Heparin is a sulfated glycosaminoglycan enriched in the h...

    Dandan Wu, **aomei Li, Yanying Yu, Bingxue Gong, **anxuan Zhou in Biotechnology Letters (2021)

  5. No Access

    Article

    Metabolomics integrated with transcriptomics: assessing the central metabolism of marine red yeast Sporobolomyces pararoseus under salinity stress

    Salinity stress is one of the most serious environmental issues in agricultural regions worldwide. Excess salinity inhibits root growth of various crops, and results in reductions of yield. It is of crucial to...

    Chunji Li, Die Zhao, Jianyu Yan, Ning Zhang, Bingxue Li in Archives of Microbiology (2021)

  6. No Access

    Article

    The construction of a dual-functional strain that produces both polysaccharides and sulfotransferases

    Heparosan is used as the starting polysaccharide sulfated using sulfotransferase to generate fully elaborate heparin, a widely used clinical drug. However, the preparation of heparosan and enzymes was consider...

    **aomei Li, Yanying Yu, Jiaqing Tang, Bingxue Gong, Wen**g Li in Biotechnology Letters (2021)

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    Article

    Long Non-coding RNA TPT1-AS1 Suppresses APC Transcription in a STAT1-Dependent Manner to Increase the Stemness of Colorectal Cancer Stem Cells

    Cancer stem cells (CSCs) are the major culprits leading to a new level of complexity and the consequential therapy resistance and disease recurrence in colorectal cancer (CRC). This study focuses on the effect...

    Bingxue Chen, Haojie Sun, Suting Xu, Qi Mo in Molecular Biotechnology (2022)

  8. No Access

    Reference Work Entry In depth

    Extracellular Vesicle-Mediated CRISPR/Cas Delivery: Their Applications in Molecular Imaging and Precision Biomedicine

    In this book chapter, we provide a comprehensive overview of the latest breakthroughs of delivery systems of CRISPR-Cas via extracellular vesicles (EVs) towards precise therapeutics and diagnosis. Typically, r...

    Dong Bingxue, Lang Wenchao, Bengang **ng in Handbook of Chemical Biology of Nucleic Acids (2023)

  9. No Access

    Living Reference Work Entry In depth

    Extracellular Vesicle-Mediated CRISPR/Cas Delivery: Their Applications in Molecular Imaging and Precision Biomedicine

    In this book chapter, we provide a comprehensive overview of the latest breakthroughs of delivery systems of CRISPR-Cas via extracellular vesicles (EVs) towards precise therapeutics and diagnosis. Typically, r...

    Dong Bingxue, Lang Wenchao, Bengang **ng in Handbook of Chemical Biology of Nucleic Acids

  10. No Access

    Article

    Simultaneous Extraction of Flavonoid Glycosides and Flavonoid Aglycones from Discarded Apple Branches by Enzyme-assisted Micelle-mediated Extraction with Cloud Point Enrichment Method

    Extraction of flavonoid glycosides and flavonoid aglycones simultaneously by enzyme-assisted micelle-mediated extraction has been proposed in this study. The discarded apple tree branches pruned during winter ...

    Yuanyuan Li, Qilei Yang, Bingxue Liu, Yanjie Liu in Food and Bioprocess Technology (2023)

  11. No Access

    Article

    Regulation of cell differentiation to promote pullulan synthesis in Aureobasidium pullulans NG

    Pullulan is a polymer produced by Aureobasidium spp. The yield of pullulan production can be impacted by the cellular differentiation of Aureobasidium spp., which changes with alterations in the growth environmen...

    Nan Zeng, Ning Zhang, Dandan Wang, Jiajia Long in Applied Microbiology and Biotechnology (2023)