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Article
Dissimilation curves as a simple method for the characterization of growth of plant cell suspension cultures
A simple non-invasive method for the characterization of growth of a plant cell suspension in a single culture flask is given. The dissimilation of sugars by a cell-culture causes a loss of weight of the conte...
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Article
Isolation of cytochrome P-450 cDNA clones from the higher plant Catharanthus roseus by a PCR strategy
Cytochrome P-450 monooxygenases are membrane-bound enzymes involved in a wide range of biosynthetic pathways in plants. An efficient PCR strategy for isolating cytochrome P-450 cDNA clones from plant cDNA libr...
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Article
Novel members of a family of AT hook-containing DNA-binding proteins from rice are identified through theirin vitro interaction with consensus target sites of plant and animal homeodomain proteins
The AT hook is an AT-rich DNA-binding domain that occurs three times in mammalian high-mobility-group I/Y chromosomal proteins and has recently also been identified in DNA-binding proteins from plants. We unex...
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Article
Characterization of the KNOX class homeobox genes Oskn2 and Oskn3 identified in a collection of cDNA libraries covering the early stages of rice embryogenesis
For identification of genes involved in embryogenesis in the model cereal rice, we have constructed a collection of cDNA libraries of well-defined stages of embryo development before, during and after organ di...
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Chapter
Yeast as Versatile Tool in Transcription Factor Research
Besides representing an important production organism in the biotechnology industry, the yeast Saccharomyces cerevisiae also constitutes a powerful organism for studying the fundamental processes operative in cel...
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Article
Glucocorticoid-inducible gene expression in rice
We have studied the use of a glucocorticoid receptor-based inducible gene expression system in the monocotyledonous model plant rice (Oryza sativa L.). This system, originally developed by T. Aoyama and N.-H. Ch...
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Article
Developmental regulation and downstream effects of the knox class homeobox genes Oskn2 and Oskn3 from rice
Plant homeobox genes of the class 1 knox (knotted1-like) type are involved in the regulation of shoot apical meristem formation and function. Their expression generally occurs either throughout the meristem or sp...
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Article
Characterization of five novel dehydration-responsive homeodomain leucine zipper genes from the resurrection plant Craterostigma plantagineum
Homeodomain leucine zipper (HDZip) genes encode putative transcription factors that are unique to plants. A function in regulating processes that are specific for plants is postulated, such as responses to enviro...
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Article
Different subcellular localization and trafficking properties of KNOX class 1 homeodomain proteins from rice
Genes of the KN1-like homeobox (KNOX) class 1 encode transcription factors involved in shoot apical meristem development and maintenance. We studied the subcellular localization of Green Fluorescent Protein-ta...
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Article
Different subcellular localization and trafficking properties of KNOX class 1 homeodomain proteins from rice
Genes of the KN1-like homeobox (KNOX) class 1 encode transcription factors involved in shoot apical meristem development and maintenance. We studied the subcellular localization of Green Fluorescent Protein-ta...
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Article
EU-OSTID: A Collection of Transposon Insertional Mutants for Functional Genomics in Rice
A collection of 1373 unique flanking sequence tags (FSTs), generated from Ac/Ds and Ac transposon lines for reverse genetics studies, were produced in japonica and indica rice, respectively. The Ds and Ac FSTs to...
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Article
A Homeodomain Leucine Zipper Gene from Craterostigma plantagineum Regulates Abscisic Acid Responsive Gene Expression and Physiological Responses
A subset of homeodomain leucine zipper proteins (HDZip) play a role in regulating adaptation responses including developmental adjustment to environmental cues in plants. Here we report the structural and func...
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Article
A genome-wide survey of HD-Zip genes in rice and analysis of drought-responsive family members
The homeodomain leucine zipper (HD-Zip) genes encode transcription factors that have diverse functions in plant development and have often been implicated in stress adaptation. The HD-Zip genes are the most ab...
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Protocol
Yeast One-Hybrid Screens for Detection of Transcription Factor DNA Interactions
The yeast one-hybrid system is widely recognized as a valuable and straightforward technique to study interactions between transcription factors and DNA. By means of one-hybrid screens, transcription factors o...
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Article
Function of the HD-Zip I gene Oshox22 in ABA-mediated drought and salt tolerances in rice
Oshox22 belongs to the homeodomain-leucine zipper (HD-Zip) family I of transcription factors, most of which have unknown functions. Here we show that the expression of Oshox22 is strongly induced by salt stress, ...