Bacteriophages
Methods and Protocols, Volume 3
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26 Result(s)
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Andrew M. Kropinski
Microbiology
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Protocol
Bacteriophage Taxonomy: A Continually Evolving Discipline
While taxonomy is an often underappreciated branch of science, it serves very important roles. Bacteriophage taxonomy has evolved from a discipline based mainly on morphology, characterized by the work of Davi...
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Open AccessGenomic and proteomic characterization of vB_SauM-UFV_DC4, a novel Staphylococcus jumbo phage
Staphylococcus aureus is one of the most relevant mastitis pathogens in dairy cattle, and the acquisition of antimicrobial resistance genes presents a significant health issue in both veterinary and human fields....
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Practical Advice on the One-Step Growth Curve
The one-step growth experiment is fundamental to the description of a new bacteriophage. The following protocol is optimized for those working with rapidly growing bacterial cultures.
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Essential Steps in Characterizing Bacteriophages: Biology, Taxonomy, and Genome Analysis
Because of the rise in antimicrobial resistance there has been a significant increase in interest in phages for therapeutic use. Furthermore, the cost of sequencing phage genomes has decreased to the point whe...
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Bacteriophage Taxonomy: An Evolving Discipline
While taxonomy is an often-unappreciated branch of science it serves very important roles. Bacteriophage taxonomy has evolved from a mainly morphology-based discipline, characterized by the work of David Bradl...
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Article
Open AccessKlebsiella phages representing a novel clade of viruses with an unknown DNA modification and biotechnologically interesting enzymes
Lytic bacteriophages and phage-encoded endolysins (peptidoglycan hydrolases) provide a source for the development of novel antimicrobial strategies. In the present study, we focus on the closely related (96 % ...
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Open AccessComparative analysis of multiple inducible phages from Mannheimia haemolytica
Mannheimia haemolytica is a commensal bacterium that resides in the upper respiratory tract of cattle that can play a role in bovine respiratory disease. Prophages are common in the M....
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Open AccessIsolation and characterisation of KP34—a novel φKMV-like bacteriophage for Klebsiella pneumoniae
Bacteriophage KP34 is a novel virus belonging to the subfamily Autographivirinae lytic for extended-spectrum β-lactamase-producing Klebsiella pneumoniae strains. Its biological features, morphology, susceptibilit...
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Open AccessClassification of Myoviridae bacteriophages using protein sequence similarity
We advocate unifying classical and genomic classification of bacteriophages by integration of proteomic data and physicochemical parameters. Our previous application of this approach to the entirely sequenced ...
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Internet Resources of Interest to Bacteriophage Workers
The Internet provides a myriad of useful tools for the phage worker including access to culture collections, specific databases, tools for gene identification, and whole genome comparisons, lecture notes, info...
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Measurement of the Bacteriophage Inactivation Kinetics with Purified Receptors
Practical methods are described for studying the interaction between bacterial viruses and their surface receptors.
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Enumeration of Bacteriophages by Double Agar Overlay Plaque Assay
The determination of the concentration of infectious phage particles is fundamental to many protocols in phage biology, genetics, and molecular biology. In this chapter the classical overlay protocol is descri...
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Approaches to the Compositional Analysis of DNA
DNA base compositional analysis is something which is rarely undertaken today, but it is still a useful criterion for phage taxonomy. A variety of techniques are described including hydrolysis of the DNA to th...
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Measurement of the Rate of Attachment of Bacteriophage to Cells
Practical methods are described for studying the adsorption rate of bacteriophages to cells and the interaction between these viruses and their surface receptors.
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Bacteriophage Enrichment from Water and Soil
Classical bacterial enrichment devised by Sergius Winogradsky (1856–1953) and Martinus Beijerinck (1851–1931) can be modified to enrich for bacteria-specific viruses. In this chapter simple protocols are prese...
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Generalized Transduction by Lytic Bacteriophages
As interest in lytic phages as antimicrobial therapies or as treatments to reduce environmental contamination with pathogenic bacteria has increased, so has the need to determine if the use of lytic phages may...
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In Sffamily Identification of Genes in Bacteriophage DNA
One of the most satisfying aspects of a genome sequencing project is the identification of the genes contained within it.These are of two types: those which encode tRNAs and those which produce proteins. After...
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