4,149 Result(s)
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Large-scale T-DNA mutagenesis in Arabidopsis for functional genomic analysis
In planta Agrobacterium-mediated transformation combined with a soil-based herbicide selection for transgenic plants was used to recover large numbers of transgenic Arabidopsis plants for functi...
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Serotype classification and characterisation of the rotavirus SA11 VP6 protein using mass spectrometry and two-dimensional gel electrophoresis
VP6, which makes up the inner capsid of rotavirus, is the major structural protein of this virus. Whilst VP6 has been sequenced at the DNA level in several rotavirus strains, there has been less effort to cha...
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An integrated web interface for large-scale characterization of sequence data
Large-scale genome projects require the analysis of large amounts of raw data. This analysis often involves the application of a chain of biology-based programs. Many of these programs are difficult to operat...
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Weeding out the genes: the Arabidopsis genome project
The Arabidopsis genome sequence is scheduled for completion at the end of this year (December 2000). It will be the first higher plant genome to be sequenced, and will allow a detailed comparison with bacterial,...
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Genes active in develo** wheat endosperm
This paper describes the construction and characterisation of a cDNA library from wheat endosperm tissue during the early stages of grain filling. Develo** wheat endosperm tissue was characterised with resp...
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A new journal for the post-genome era
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Transactivation of a target gene through feedforward loop activation in plants
The expression of many genes encoding transcriptional activators in prokaryotes and eukaryotes is upregulated through positive feedback activation. During positive feedback activation, a transcriptional activ...
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The stability of thermophilic proteins: a study based on comprehensive genome comparison
We address the question of the thermal stability of proteins in thermophiles through comprehensive genome comparison, focussing on the occurrence of salt bridges. We compared a set of 12 genomes (from four th...
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Comparison of functional annotation schemes for genomes
In this paper we survey a number of functional classification schemes applicable to genomes. We present the concepts of depth, breadth and resolution as descriptors of the schemes' scope and architecture and ...
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Gene therapy – when a simple concept meets a complex reality
In theory, gene therapy is a simple concept which holds great promise as a cure for disease. In practice, however, considerable obstacles have to be overcome, including problems with safe and efficient gene d...
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Map** a new frontier; development of integrated cytogenetic maps in plants
Cytogenetic maps, as the name implies, incorporate data from genetic maps with actual cytological features of chromosomes such as centromeres, knobs and, recently, fluorescence in situ hybridization (FISH) si...
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Gene expression changes associated with cytotoxicity identified using cDNA arrays
In order to investigate gene expression changes associated with cytotoxicity, we used cDNA arrays to monitor the expression of over 5,000 genes in response to toxic stress in the HepG2 liver cell line. Cells ...
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Societal and Medical Consequences of the Human Genome Project
The Human Genome Project is a US-based molecular biological project, the results of which are likely to be implemented on humans. The sociopolitical dimension of this is highly neglected. The aim of the confe...
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Functional analysis of the yeast genome
Just as Saccharomyces cerevisiae itself provides a model for so many processes essential to eukaryotic life, we anticipate that the methods and the mindset that have moved yeast biological research "beyond the g...
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ES cell neural differentiation reveals a substantial number of novel ESTs
We have used a method for synchronously differentiating murine embryonic stem (ES) cells into functional neurons and glia in culture. Using subtractive hybridization we isolated approximately 1200 cDNA clones...
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Social responsibility in human genetics – a survey
The potential for molecular biological manipulation of human beings is a challenge to the social responsibility of human geneticists. The concept of individual healthcare is compromised by a reductionist appr...
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Social and medical consequences of the human genome project
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Assessing reliability of gene clusters from gene expression data
The rapid development of microarray technologies has raised many challenging problems in experiment design and data analysis. Although many numerical algorithms have been successfully applied to analyze gene ...
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Dynamic architecture of the yeast cell cycle uncovered by wavelet decomposition of expression microarray data
Wavelet analysis has been applied to yeast cell cycle expression microchip data to reveal large-scale temporal structures and ubiquitous oscillations in mRNA levels. Discrete intervals in time within the cell...
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