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Biochemistry, general
Bioinformatics
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Article
Efficacy of methylchloro/methylisothiazolone biocide againstLegionella pneumophila in cooling tower water
Methylchloro/methylisothiazolone biocide was tested for efficacy in cooling tower water againstLegionella pneumophila (strains Flint 1 and 2) andL. gormanii. These analyses are the first reports on the efficacy o...
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Article
Effect of carbohydrate source on alpha-amylase and glucoamylase formation byClostridium acetobutylicum SA-1
An examination into the effect of different carbohydrate sources indicated that the production of extracellular alpha-amylase and glucoamylase was under similar biosynthetic control inClostridium acetobutylicum S...
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Article
Construction of a new vector for the expression of foreign genes inZymomonas mobilis
Broad host range plasmids have previously been shown to be suitable as vectors to introduce antibiotic resistance genes intoZ. mobilis. However, attempts to use these vectors to carry other genes with enteric pro...
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Degradation of highly chlorinated PCBs byPseudomonas strain LB400
Congeners of polychlorinated biphenyl (PCB) differ in the number and position of chlorine substituents. Although PCBs are degraded, those congoners with five or more chlorines have been considered resistant to...
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Article
Physiological and genetic factors for process development of cyclosporine fermentations
The new immunosuppressive agent cyclosporine (Cyclosporin A, Cy) is the most prominent member of a group of cyclic peptide fungal metabolites (cyclosporins) produced byTolypocladium inflatum in submerged fermenta...
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Article
Phenylacetic acid in an anaerobic swine manure digester
The presence of phenylacetic acid (PAA) in an anaerobic swine manure digester was determined by gas chromatography of the butyl ester and confirmed by mass spectroscopy. PAA concentration increased during star...
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Article
Alteration of some toxic aflatoxin responses in Syrian hamsters fed zinc carbonate-supplemented diets
Chronic exposure to aflatoxins (AFTs) below the LD50 can result in reduced weight gain, hepatocellular necrosis and bile duct cell proliferation. Here, we report whether dietary zinc (Zn2+) protects against both ...
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Article
Celebration
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Article
Enzymic nucleotidylylation of lincosaminide antibiotics
Fermentations ofStreptomyces coelicolor are known to convert lincosaminide antibiotics to mixtures of their inactive 3-(5′-ribonucleotides). In the present study, lincomycin, clindamycin and pirlimycin were nucle...
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Article
Biodegradation of xanthan by salt-tolerant aerobic microorganisms
Three salt-tolerant bacteria which degraded xanthan were isolated from various water and soil samples collected from New Jersey, Illinois, and Louisiana. The mixed culture, HD1, contained aBacillus sp. which prod...
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Article
Absence or presence of glucose in growth medium and its effect on heat injury inStaphylococcus aureus
Staphylococcus aureus 196E, when grown in a glucose (≥0.25% wt./vol.)-containing medium, produced cells that would undergo injury when subjected to sublethal heat conditions (45 min at 50°C); however, if glucose...
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Article
The effect of fifteen biocides on formaldehyde-resistant strains ofPseudomonas aeruginosa
Evaluation of formaldehyde and fifteen biocides in formaldehyde sensitive (S) and resistant (R) strains ofPseudomonas aeruginosa revealed a pattern of response that allowed a comparison of the mode of action of t...
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Article
Oxidation of propene and 1-butene byMethylococcus capsulatus andMethylosinus trichosporium
Methane-grown cells ofMethylococcus capsulatus andMethylosinus trichosporium readily oxidized propene and various isomers of butene to their respective epoxides. When examined in a proton NMR spectrum using tris(...
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Article
The mechanism of action of hexahydro-1,3,5-triethyl-s-triazine
The mechanism of antimicrobial action of hexahydro-1,3,5-triethyl-s-triazine (HHTT) was studied using the HHTT-resistant isolate,Pseudomonas putida 3-T-152, its HHTT-sensitive, novobiocin-cured derivative,P. puti...
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Article
Erratum
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Article
Synthesis of unique proteins at the onset of carbon starvation inEscherichia coli
Escherichia coli bulk protein synthesis continued during the first 3–4 h of carbon starvation at 50–75% that of non-starved (growing) cells. Two-dimensional gel electrophoresis analysis of in vivo pulse-labelled...
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Article
Cloning of an endoglucanase gene fromPseudomonas fluorescens var.cellulosa intoEscherichia coli andPseudomonas fluorescens
An endoglucanase chromosomal gene from the cellulolyticPseudomonas fluorescens var.cellulosa (NCIB 10462) was cloned inEscherichia coli. Chromosomal DNA was partially digested with the restriction enzymeEcoRI and...
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Article
Induction and selection of formaldehyde-based resistance inPseudomonas aeruginosa
A formaldehyde resistant (R) phenotype ofPseudomonas aeruginosa was isolated from a formaldehydesensitive (S) parent by sequential treatment with 1,3,5-tris-(ethyl)hexahydro-s-triazine (ET). The resistance of the...
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Article
The use of genetic probes to detect microorganisms in biomining operations
Microorganisms are currently used for the recovery of copper from mining dumps of low-grade ore. One of the most important microorganisms involved in copper-solubilization isThiobacillus ferrooxidans, although ma...
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Article
The mechanism of microbial resistance to hexahydro-1,3,5-triethyl-s-triazine
Four strains ofPseudomonas putida and two unidentifiedPseudomonas species that were resistant to hexahydro-1,3,5-triethyl-s-triazine (HHTT) were shown to be resistant to formaldehyde as well. Conjugation experime...
