15 Result(s)
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**ongxiong He
Biotechnology
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Article
Elimination of editing plasmid mediated by theophylline riboswitch in Zymomonas mobilis
Zymomonas mobilis is regarded as a potential chassis for the production of platform chemicals. Genome editing using the CRISPR-Cas system could meet the need for gene modification in metabolic engineering. Howev...
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Open AccessMolecular mechanism of engineered Zymomonas mobilis to furfural and acetic acid stress
Acetic acid and furfural (AF) are two major inhibitors of microorganisms during lignocellulosic ethanol production. In our previous study, we successfully engineered Zymomonas mobilis 532 (ZM532) strain by genome...
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Taraxasterol Inhibits Hyperactivation of Macrophages to Alleviate the Sepsis-induced Inflammatory Response of ARDS Rats
To explore the effect and mechanism of taraxasterol on sepsis-induced acute respiratory distress syndrome (ARDS). Twenty-four male SD rats were randomly divided into four groups: the control group, model (lipo...
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Open Accessd-Lactic acid production from agricultural residues by membrane integrated continuous fermentation coupled with B vitamin supplementation
d-Lactic acid played an important role in the establishment of PLA as a substitute for petrochemical plastics. But, so far, the d-lactic acid production was limited in only pilot scale, which was definitely unabl...
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Open AccessCellulosic ethanol production by consortia of Scheffersomyces stipitis and engineered Zymomonas mobilis
As one of the clean and sustainable energies, lignocellulosic ethanol has achieved much attention around the world. The production of lignocellulosic ethanol does not compete with people for food, while the co...
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Open AccessEndogenous CRISPR-assisted microhomology-mediated end joining enables rapid genome editing in Zymomonas mobilis
Zymomonas mobilis is a natural ethanologen with many desirable characteristics, making it an ideal platform for future biorefineries. Recently, an endogenous CRISPR-based genome editing tool has been developed fo...
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Open AccessDevelopment and characterization of acidic-pH-tolerant mutants of Zymomonas mobilis through adaptation and next-generation sequencing-based genome resequencing and RNA-Seq
Acid pretreatment is a common strategy used to break down the hemicellulose component of the lignocellulosic biomass to release pentoses, and a subsequent enzymatic hydrolysis step is usually applied to releas...
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Novel mutagenesis and screening technologies for food microorganisms: advances and prospects
Microorganisms are indispensable in the food industry, but wild-type strains hardly meet the current industrial demands due to several undesirable traits. Therefore, microbial strain improvement offers a criti...
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Open AccessGenome shuffling enhances stress tolerance of Zymomonas mobilis to two inhibitors
Furfural and acetic acid are the two major inhibitors generated during lignocellulose pretreatment and hydrolysis, would severely inhibit the cell growth, metabolism, and ethanol fermentation efficiency of Zymomo...
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Bio-detoxification Bacteria Isolated from Dye-Polluted Soils Promote Lactic Acid Production from Ammonia Pretreated Corn Stover
Agro-stovers are the most abundant substrates for producing lactic acid, which has great potential application in the production of biodegradable and biocompatible polylactic acid polymers. However, chemical p...
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Open AccessReplacing water and nutrients for ethanol production by ARTP derived biogas slurry tolerant Zymomonas mobilis strain
Reducing fresh water consumption and nutrient addition will be an effective way to reduce the whole cost of bioethanol production. On the other hand, treatment of biogas slurry derived from anaerobic digestion...
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Open AccessEngineered Zymomonas mobilis tolerant to acetic acid and low pH via multiplex atmospheric and room temperature plasma mutagenesis
Cellulosic biofuels are sustainable compared to fossil fuels. However, inhibitors, such as acetic acid generated during lignocellulose pretreatment and hydrolysis, would significantly inhibit microbial ferment...
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Open AccessSingle-cell Protein and Xylitol Production by a Novel Yeast Strain Candida intermedia FL023 from Lignocellulosic Hydrolysates and Xylose
Yeasts are good candidates to utilize the hydrolysates of lignocellulose, the most abundant bioresource, for bioproducts. This study aimed to evaluate the efficiencies of single-cell protein (SCP) and xylitol ...
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Bacillus vini sp. nov. isolated from alcohol fermentation pit mud
A novel aerobic, Gram-stain-positive, sporogenous, rod-shaped bacterium, designated LAM0415T, was isolated from an alcohol fermentation pit mud sample collected from Sichuan Luzhou-flavour liquor enterprise in Ch...
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Open AccessUsing global transcription machinery engineering (gTME) to improve ethanol tolerance of Zymomonas mobilis
With the increasing global crude oil crisis and resulting environmental concerns, the production of biofuels from renewable resources has become increasingly important. One of the major challenges faced during...
