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  1. No Access

    Chapter

    Biomanufacturing by In Vitro Biotransformation (ivBT) Using Purified Cascade Multi-enzymes

    In vitro biotransformation (ivBT) refers to the use of an artificial biological reaction system that employs purified enzymes for the one-pot conversion of low-cost materials into biocommodities such as ethano...

    Yanmei Qin, Qiangzi Li, Lin Fan, **ao Ning, **nlei Wei, Chun You in Cell-free Production (2023)

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    Article

    An ATP-free in vitro synthetic enzymatic biosystem facilitating one-pot stoichiometric conversion of starch to mannitol

    D-Mannitol (hereinafter as mannitol) is a six-carbon sugar alcohol with diverse applications in food and pharmaceutical industries. To overcome the drawbacks of the chemical hydrogenation method commonly used ...

    **nlei Wei, Qiangzi Li, Congcong Hu, Chun You in Applied Microbiology and Biotechnology (2021)

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    Article

    Enzymatic characterization of a thermostable phosphatase from Thermomicrobium roseum and its application for biosynthesis of fructose from maltodextrin

    Phosphatases, which catalyze the dephosphorylation of compounds containing phosphate groups, are important members of the haloacid dehalogenase (HAD)–like superfamily. Herein, a thermostable phosphatase encode...

    Dongdong Meng, Ailing Liang, **nlei Wei, Chun You in Applied Microbiology and Biotechnology (2019)