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    Direct Cloning of Full-Length Cell Differentially Expressed Genes by Multiple Rounds of Subtractive Hybridization Based on Long-Distance PCR and Magnetic Beads

    Subtractive cloning is an ideal technique for identifying genes differentially expressed in two nuclear acids population (1). The polymerase chain reaction (PCR)-based subtraction is the method of choice when the...

    **n Huang, Zhenglong Yuan, Xuetao Cao in PCR Cloning Protocols (2002)