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Article
The modification of heme special importer to improve the production of active hemoglobins in Escherichia coli
To enhance the import of heme for the production of active hemoproteins in Escherichia coli C41 (DE3) lacking the special heme import system, heme receptor ChuA from E. coli Nissle 1917 was modified through molec...
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Article
Investigation of New Integrated Drought Monitoring Model Taking into Account the Effects of Climate Anomalies
Drought mechanisms vary markedly within different ecogeographical regions. Existing drought indices do not reflect the impact of climate anomalies on drought. In this study, the climate anomaly index was incor...
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Article
Open AccessHabitat radiomics and deep learning fusion nomogram to predict EGFR mutation status in stage I non-small cell lung cancer: a multicenter study
Develop a radiomics nomogram that integrates deep learning, radiomics, and clinical variables to predict epidermal growth factor receptor (EGFR) mutation status in patients with stage I non-small cell lung can...
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Article
Reflections on appropriately liberalizing ART for groups requiring special attention in China
The continuous advancement of assisted reproductive technologies (ART) and the evolving attitudes towards marriage and fertility among the general public have led to an increasing number of groups requiring sp...
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Article
Construction of short synthetic promoters for optimization of ovalbumin expression level in Saccharomyces cerevisiae
Saccharomyces cerevisiae, a common eukaryotic model microorganism that is generally recognized as safe, is widely employed for the heterologous expression of edible and therapeutic proteins. Promoters are crucial...
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Article
Construction of a plasmid-free Escherichia coli strain for lacto-N-neotetraose biosynthesis
Lacto-N-neotetraose (LNnT), with promising bioactive properties, is one of the most significant nonfucosylated human milk saccharides. Recently, its synthesis by microbial fermentation has attracted great attenti...
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Article
Construction of immobilized enzyme cascades for the biosynthesis of nucleotide sugars UDP-N-acetylglucosamine and UDP-glucuronic acid
UDP-sugars, as active forms of monosaccharides, play integral roles in glycosylation and biosynthesis of polysaccharides. Although enzymatic catalysis has achieved great process, the comparatively low producti...
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Article
Open AccessAttentional impairment and altered brain activity in healthcare workers after mild COVID-19
Severe acute respiratory syndrome coronavirus 2 (SARS-COV-2) is highly transmissible and pathogenic. Patients with mild cases account for the majority of those infected with coronavirus disease 2019 (COVID-19)...
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Article
Metabolic engineering of Bacillus subtilis for de novo synthesis of 6′-sialyllactose
6′-sialyllactose (6′-SL) is an important component of human milk oligosaccharides (HMOs) and has numerous infant health benefits. The construction of efficient and food-safe microbial cell factories to produce 6′
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Article
Open AccessInternal water circulation mediated synergistic co-hydrolysis of PET/cotton textile blends in gamma-valerolactone
Recycling strategies for mixed plastics and textile blends currently aim for recycling only one of the components. Here, we demonstrate a water coupling strategy to co-hydrolyze polyester/cotton textile blends...
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Article
Open AccessSerum cytokines and creatinine/cystatin C ratio as prognostic biomarkers in advanced cancer patients treated with anti-PD-1/PD-L1 therapy
Immune checkpoint inhibitors (ICIs), specifically targeting the programmed cell death protein-1 or its ligand (PD-1/PD-L1), have been extensively used in the treatment of a spectrum of malignancies, although t...
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Article
Open AccessShort-term effects of irrigation and nitrogen management on paddy soil carbon pools under deep placement of basal fertilizer nitrogen
Active soil organic carbon (SOC) fractions are major driving factors of soil fertility. Understanding the effects of water and fertilizer management on changes in active SOC fractions helps improve soil qualit...
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Article
Open AccessMachine learning models for abstract screening task - A systematic literature review application for health economics and outcome research
Systematic literature reviews (SLRs) are critical for life-science research. However, the manual selection and retrieval of relevant publications can be a time-consuming process. This study aims to (1) develop...
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Article
Seismic displacement of bedding slopes stabilized with anchor cables and piles considering dynamic yield acceleration
Seismic displacements are critical analysis components for evaluating the slope stability during earthquakes and essentially regulated by the yield acceleration of slopes. The conventional Newmark’s method app...
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Article
Improvement of membrane stabilizer on the rehydrated reconstruction of spray-dried mannitol-based liposome powder
Spray drying is regarded as one of the most economical methods utilized in the production of liposome powder in industry. However, the poor reconstruction of the powder following rehydration imposes constraint...
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Article
A process-based inherent strain method for prediction of deformation and residual stress for wire-arc directed energy deposition
Accurate and efficient prediction of deformation and residual stress in large metal components manufactured through wire-arc directed energy deposition is essential for optimizing the process parameters and en...
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Article
Optimization of linear plasmid expression system for protein production and secretion in Bacillus thuringiensis
Bacillus thuringiensis, a safe bacterium widely used in agriculture for the biocontrol of pests, has great potential for protein production. The linear plasmid expression system, bacterial orthogonal DNA replicat...
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Article
Open Access50% efficacy dose of intravenous lidocaine in supressing sufentanil-induced cough in children: a randomised controlled trial
Opioids such as sufentanil are used as anaesthetics due to their rapid action and superior analgesic effect. However, sufentanil induces a huge cough in paediatric patients. In contrast, intravenous (IV) lidoc...
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Article
Individual and Community level Developmental Adversities: Associations with Marijuana and Alcohol Use in Late-Adolescents and Young Adults
Exposure to community and individual level stressors during adolescence has been reported to be associated with increased substance use. However, it remains unclear what the relative contribution of different ...
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Article
Open AccessDiagnostic performance of a rapid immunochromatographic test for the simultaneous detection of antibodies to Theileria equi and Babesia caballi in horses and donkeys
Equine piroplasmosis is caused by two tick-borne protozoan parasites, Theileria equi and Babesia caballi,, which are clinically relevant in susceptible horses, donkeys, and mules. Moreover, equine piroplasmosis s...
