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CRISPR/Cas9-Mediated Genome Editing in Indica Rice (Oryza sativa L. subsp. indica var. CR-5272)
Tissue culture optimization protocols limit indica rice breeding. Such a challenge is vital because emergent techniques still rely on tissue culture...
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A Comprehensive Protocol for Assembly of Multiple gRNAs into a Direct Vector for Genome Editing in Tomato
The Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)—Cas 9 (CRISPR-associated protein 9) is a robust DNA-encoded, RNA-mediated...
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Cytogenetic Bioindication in Root Meristems for Vitality Assessment of Trees
Our method describes how to collect forest tree root tips in the field, to store them for transfer to the lab, to pretreat root tips in order to...
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Genome Editing in Brassica juncea Using CRISPR/Cas9 Technology
Modern genome editing tools particularly CRISPR/Cas9 have revolutionized plant genome manipulation for engineering resilience against changing...
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Measurement of O2 Uptake and Evolution in Leaves In Vivo Using Stable Isotopes and Membrane Inlet Mass Spectrometry
Oxygen is both product and substrate of photosynthesis and metabolism in plants, by oxygen evolution through water splitting and uptake by...
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Carotenoid Extraction from Plant Tissues
Carotenoids are the natural pigments available in nature and exhibit different colors such as yellow, red, and orange. These are a class of...
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A Beginner’s Guide to Eddy Covariance: Methodology and Its Applications to Photosynthesis
The eddy covariance technique, commonly applied using flux towers, enables the investigation of greenhouse gas (e.g., carbon dioxide, methane,...
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In Silico Models to Validate Novel Blood-Based Biomarkers
Biological validation of preliminary findings is a key prerequisite in biomarker discovery. In recent years, the development of advanced large-scale...
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Alzheimer’s Disease Prevention and Treatment Based on Population-Based Approaches
The development of effective prevention and treatment strategies for Alzheimer’s disease (AD) and dementia is hindered by limited knowledge of the...
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Imaging Cell Signaling in Tissues Using the IBEX Method
The study of cell signaling within tissues can be enhanced using highly multiplexed immunohistochemistry to localize the presence and spatial...
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CSF N-Glycomics Using MALDI MS Techniques
In this chapter, we will present the methodology currently applied in our laboratory for the structural elucidation of the cerebrospinal fluid (CSF)...
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High-Throughput Lipidomic and Metabolomic Profiling for Brain Tissue and Biofluid Samples in Neurodegenerative Disorders
Recent research has revealed the potential of lipidomics and metabolomics in identifying new biomarkers and mechanistic insights for...
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In Situ Imaging of Proteins Using DNA-PAINT Super-Resolution Microscopy
The spatial resolution of conventional light microscopy is restricted by the diffraction limit to hundreds of nanometers. Super-resolution microscopy...
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Deep Learning-Based Cell Tracking in Deforming Organs and Moving Animals
Cell tracking is an essential step in extracting cellular signals from moving cells, which is vital for understanding the mechanisms underlying...
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CSF N-Glycomics Using High-Throughput UPLC-ESI Techniques in Alzheimer’s Disease
In this chapter, we will present a high-throughput method applied in our laboratory for the structural elucidation of the cerebrospinal fluid (CSF)...
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Single-Cell Measurements Using Acoustic Force Spectroscopy (AFS)
Single-molecule force spectroscopy is a powerful tool to investigate the forces and motions related to interactions of biological molecules. Acoustic...
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Lattice Light-Sheet Motor-PAINT: A Method to Map the Orientations of Microtubules in Complex Three-Dimensional Arrays
Microtubules play an essential role in many cellular functions, in part by serving as tracks for intracellular transport by kinesin and dynein. The...
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Insect Cell-Based Expression of Cytoskeletal Motor Proteins for Single-Molecule Studies
Cytoskeletal motor proteins are essential molecular machines that hydrolyze ATP to generate force and motion along cytoskeletal filaments. Members of...
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Atomic Force Microscopy: An Introduction
Imaging of nano-sized particles and sample features is crucial in a variety of research fields, for instance, in biological sciences, where it is...
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In Vivo Optogenetic Phase Transition of an Intrinsically Disordered Protein
Proteins containing intrinsically disordered regions (IDRs) control a wide variety of cellular processes by assembly of membrane-less organelles via...