Search
Search Results
-
Alzheimer’s Disease Prevention and Treatment Based on Population-Based Approaches
The development of effective prevention and treatment strategies for Alzheimer’s disease (AD) and dementia is hindered by limited knowledge of the...
-
CSF N-Glycomics Using MALDI MS Techniques
In this chapter, we will present the methodology currently applied in our laboratory for the structural elucidation of the cerebrospinal fluid (CSF)...
-
High-Throughput Lipidomic and Metabolomic Profiling for Brain Tissue and Biofluid Samples in Neurodegenerative Disorders
Recent research has revealed the potential of lipidomics and metabolomics in identifying new biomarkers and mechanistic insights for...
-
In Situ Imaging of Proteins Using DNA-PAINT Super-Resolution Microscopy
The spatial resolution of conventional light microscopy is restricted by the diffraction limit to hundreds of nanometers. Super-resolution microscopy...
-
Imaging Cell Signaling in Tissues Using the IBEX Method
The study of cell signaling within tissues can be enhanced using highly multiplexed immunohistochemistry to localize the presence and spatial...
-
Deep Learning-Based Cell Tracking in Deforming Organs and Moving Animals
Cell tracking is an essential step in extracting cellular signals from moving cells, which is vital for understanding the mechanisms underlying...
-
CSF N-Glycomics Using High-Throughput UPLC-ESI Techniques in Alzheimer’s Disease
In this chapter, we will present a high-throughput method applied in our laboratory for the structural elucidation of the cerebrospinal fluid (CSF)...
-
Meta-analysis of Map** Studies: Integrating QTLs Towards Candidate Gene Discovery
Meta-analysis consists of pooling results from multiple studies, and allows prediction of more precise and meaningful data. A plethora of QTL map**...
-
Map** of Quantitative Traits Loci: Harnessing Genomics Revolution for Dissecting Complex Traits
With the onset of the genomic era, map** of Quantitative Traits Loci (QTLs) appears to be a feasible solution for dissecting the complex...
-
Assembly, Annotation and Visualization of NGS Data
The next-generation sequencing technologies have revolutionized the field of agriculture with the advent of ever-evolving chemistries and a reduction...
-
Overview of the Bioinformatics Databases and Tools for Genome Research and Crop Improvement
The advancement of sequencing technologies and molecular techniques has generated a huge amount of biological data which needs to be analyzed and...
-
Statistical and Biological Data Analysis Using Programming Languages
Rapid progress in high throughput sequencing technologies has generated an unprecedented huge amount of complex and heterogeneous biological data and...
-
Trait Based Association Map** in Plants
Genome-wide association studies (GWAS) estimate the likelihood of association between genotype and phenotype in any large collection of material and...
-
Statistical and Quantitative Genetics Studies
Quantitative genetics and plant breeding are non-exclusive and these disciplines has been benefited from each other for the past 100 years. As a...
-
CRISPR/Cas9 Vector Construction for Gene Knockout
This protocol outlines the construction of a plant transformation plasmid to express both the Cas9 nuclease and individual guide RNA (gRNA),...
-
A Comprehensive Protocol for Assembly of Multiple gRNAs into a Direct Vector for Genome Editing in Tomato
The Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)—Cas 9 (CRISPR-associated protein 9) is a robust DNA-encoded, RNA-mediated...
-
Genome Editing in Brassica juncea Using CRISPR/Cas9 Technology
Modern genome editing tools particularly CRISPR/Cas9 have revolutionized plant genome manipulation for engineering resilience against changing...
-
A General Protocol for Gene Expression Analysis in Chemically Mutant Coffee Plants in Response to Rust (Hemileia vastatrix Berk & Broome) Infection
Coffea spp. is the source of one of the most widely consumed beverages in the world. However, the cultivation of this crop is threatened by Hemileia...
-
Measurement of O2 Uptake and Evolution in Leaves In Vivo Using Stable Isotopes and Membrane Inlet Mass Spectrometry
Oxygen is both product and substrate of photosynthesis and metabolism in plants, by oxygen evolution through water splitting and uptake by...
-
Carotenoid Extraction from Plant Tissues
Carotenoids are the natural pigments available in nature and exhibit different colors such as yellow, red, and orange. These are a class of...