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Droplet Microfluidics with Capillary Electrophoresis for Glycan Biomarker Analysis
Several glycoproteins are validated biomarkers of various diseases such as cancer, cardiovascular diseases, chronic alcohol abuse, or congenital...
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Microchip for Immunomagnetic Sorting of Circulating Tumor Cells (CTCs)
Circulating tumor cells (CTCs) isolated directly from whole blood opens new perspectives for cancer monitoring and the development of personalized...
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Single-Cell Measurements Using Acoustic Force Spectroscopy (AFS)
Single-molecule force spectroscopy is a powerful tool to investigate the forces and motions related to interactions of biological molecules. Acoustic...
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Unfolding and Refolding Proteins Using Single-Molecule AFM
Single-molecule atomic force microscopy (AFM) allows capturing the conformational dynamics of an individual molecule under force. In this chapter, we...
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Genetic Network Design Automation with LOICA
Genetic design automation (GDA) is the use of computer-aided design (CAD) in designing genetic networks. GDA tools are necessary to create more...
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Multimodal Control of Bacterial Gene Expression by Red and Blue Light
By applying sensory photoreceptors, optogenetics realizes the light-dependent control of cellular events and state. Given reversibility,...
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The Causes for Genomic Instability and How to Try and Reduce Them Through Rational Design of Synthetic DNA
Genetic engineering has revolutionized our ability to manipulate DNA and engineer organisms for various applications. However, this approach can lead...
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High-Throughput Spectroscopic Analysis of mRNA Cap** Level
Eukaryotic mRNAs are characterized by terminal 5′ cap structures and 3′ polyadenylation sites, which are essential for posttranscriptional...
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Multiplex Functional Characterization of Protein Variant Libraries in Mammalian Cells with Single-Copy Genomic Integration and High-Throughput DNA Sequencing
Sequencing-based, massively parallel genetic assays have enabled simultaneous characterization of the genotype–phenotype relationships for libraries...
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Combining a Base Deaminase Mutator with Phage-Assisted Evolution
Phage-assisted evolution has emerged as a powerful technique for improving a protein’s function by using mutagenesis and selective pressure. However,...
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Generation of a Naïve Human scFv Phage Display Library and Panning Selection
Phage display antibody libraries have been successfully used as the essential tool to produce monoclonal antibodiesMonoclonal antibodies (mAB)...
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Single-Molecule FRET-Resolved Protein Dynamics – from Plasmid to Data in Six Steps
Single-molecule Förster resonance energy transfer (smFRET) is a powerful technique for the detection of conformational dynamics of biomolecules....
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Measuring Transcription Dynamics of Individual Genes Inside Living Cells
Transcription is a highly dynamic process, which, for many genes, occurs in stochastic bursts. Studying what regulates these stochastic bursts...
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Genome Editing, Transcriptional Regulation, and Forward Genetic Screening Using CRISPR-Cas12a Systems in Yarrowia lipolytica
Class II Type V endonucleases have increasingly been adapted to develop sophisticated and easily accessible synthetic biology tools for genome...
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Cell-Free Synthesis and Quantitation of Bacteriophages
Cell-free transcription-translation (TXTL) enables achieving an ever-growing number of applications, ranging from the rapid characterization of DNA...
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Posttranslational Remote Control Mediated by Physical Inducers for Rapid Protein Release in Engineered Mammalian Cells
Physical cues such as light, heat, or an electrical field can be utilized for traceless, on-demand activation of the expression of a desired...
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A Computational Modeling Approach for the Design of Genetic Control Systems that Respond to Transcriptional Activity
Recent progress in synthetic biology has enabled the design of complex genetic circuits that interface with innate cellular functions, such as gene...
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Imaging S-Adenosyl Methionine Dynamics in Living Cells Using an RNA-Based Fluorescent Sensor
S-Adenosyl methionine (SAM) is a critical metabolite involved in numerous cellular processes, including DNA methylation and gene expression...
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Standard Solutions and Media Used for Isolation of Soil Protists
Protozoa are heterotrophic, nonfilamentous protists which play a major role in food web decomposition as bacterivores. In both terrestrial and...
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Axenic Cultivation of Soil Protists
Most of the physiological work on the protozoa has been restricted because the organism which need to be investigated cannot be raised in pure...