Search
Search Results
-
A General Protocol for Gene Expression Analysis in Chemically Mutant Coffee Plants in Response to Rust (Hemileia vastatrix Berk & Broome) Infection
Coffea spp. is the source of one of the most widely consumed beverages in the world. However, the cultivation of this crop is threatened by Hemileia...
-
CRISPR/Cas9-Mediated Genome Editing in Indica Rice (Oryza sativa L. subsp. indica var. CR-5272)
Tissue culture optimization protocols limit indica rice breeding. Such a challenge is vital because emergent techniques still rely on tissue culture...
-
Carotenoid Extraction from Plant Tissues
Carotenoids are the natural pigments available in nature and exhibit different colors such as yellow, red, and orange. These are a class of...
-
Cytogenetic Bioindication in Root Meristems for Vitality Assessment of Trees
Our method describes how to collect forest tree root tips in the field, to store them for transfer to the lab, to pretreat root tips in order to...
-
Identification of Novel Induced Mutations in Seed and Vegetatively Propagated Plants from Reduced Representation or Whole Genome Sequencing Data
Treatment of plants with chemical mutagens results primarily in the production of novel single nucleotide variants. Mutagenesis is a mostly random...
-
CRISPR/Cas9 Vector Construction for Gene Knockout
This protocol outlines the construction of a plant transformation plasmid to express both the Cas9 nuclease and individual guide RNA (gRNA),...
-
A Comprehensive Protocol for Assembly of Multiple gRNAs into a Direct Vector for Genome Editing in Tomato
The Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)—Cas 9 (CRISPR-associated protein 9) is a robust DNA-encoded, RNA-mediated...
-
Using Magnetic Fields to Enhance the Seed Germination, Growth, and Yield of Plants
Geomagnetic field (GMF) protects living organisms on the Earth from the radiation coming from space along with other environmental factors during...
-
Genome Editing in Brassica juncea Using CRISPR/Cas9 Technology
Modern genome editing tools particularly CRISPR/Cas9 have revolutionized plant genome manipulation for engineering resilience against changing...
-
Overview of the Bioinformatics Databases and Tools for Genome Research and Crop Improvement
The advancement of sequencing technologies and molecular techniques has generated a huge amount of biological data which needs to be analyzed and...
-
Statistical and Quantitative Genetics Studies
Quantitative genetics and plant breeding are non-exclusive and these disciplines has been benefited from each other for the past 100 years. As a...
-
Trait Based Association Map** in Plants
Genome-wide association studies (GWAS) estimate the likelihood of association between genotype and phenotype in any large collection of material and...
-
Assembly, Annotation and Visualization of NGS Data
The next-generation sequencing technologies have revolutionized the field of agriculture with the advent of ever-evolving chemistries and a reduction...
-
Meta-analysis of Map** Studies: Integrating QTLs Towards Candidate Gene Discovery
Meta-analysis consists of pooling results from multiple studies, and allows prediction of more precise and meaningful data. A plethora of QTL map**...
-
Statistical and Biological Data Analysis Using Programming Languages
Rapid progress in high throughput sequencing technologies has generated an unprecedented huge amount of complex and heterogeneous biological data and...
-
Map** of Quantitative Traits Loci: Harnessing Genomics Revolution for Dissecting Complex Traits
With the onset of the genomic era, map** of Quantitative Traits Loci (QTLs) appears to be a feasible solution for dissecting the complex...
-
Sample Preparation and Phosphopeptide Enrichment for Plant Phosphoproteomics via Label-Free Mass Spectrometry
Phosphopeptide enrichment is the main bottleneck of every phosphorylation study. Therefore, in this chapter, a general workflow tries to overbridge...
-
Protein-Protein Interactions Visualized by Bimolecular Fluorescence Complementation in Arabidopsis thaliana Protoplasts from Leaf
Bimolecular fluorescence complementation (BiFC) is a powerful tool for studying protein-protein interactions in living cells. By fusing interacting...
-
Root Hair Imaging Using Confocal Microscopy
Plant genetics plays a key role in determining root hair initiation and development. A complex network of genetic interactions therefore closely...
-
Gene Co-expression Network Analysis to Mine Genes Involved in Plant Secondary Metabolite Biosynthesis and Regulation
Plants produce diverse specialized metabolites (SMs) that do not participate in plant growth and development but help them adapt to various...