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Standard Solutions and Media Used for Isolation of Soil Protists
Protozoa are heterotrophic, nonfilamentous protists which play a major role in food web decomposition as bacterivores. In both terrestrial and...
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Axenic Cultivation of Soil Protists
Most of the physiological work on the protozoa has been restricted because the organism which need to be investigated cannot be raised in pure...
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TEM: Sample Preparation, Fixation, and Staining of Protists
Transmission electron microscopy (TEM) is an ideal device to study the internal structure of cells and different types of biological materials, as...
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DNA Extraction from Trophozoites and Cysts Using Manual Protocol
Protist taxa are ubiquitous members among soil microbial communities. Soil protistan communities are very diverse and yet to be explored due to the...
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Media Used for Enrichment of Soil Protists
The enrichment of protists highly depends upon the chemical composition of the synthetic medium next to the temperature. It affects growth and...
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Enumeration of Testate Amoeba Through Direct Count from Soil
Several methods have been used to extract testate amoebae from various ecological niche to examine and quantify their tests using light microscopy....
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Most Probable Number (MPN) Method for Enumeration of Soil Protists
The most probable number (MPN) technique has been widely used to enumerate environmental microorganisms, including soil protists. This...
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Soil Sampling, Processing, and Storage
Soil is a natural element that presents unique characteristics, and soil analysis has been the most used technique for diagnosing soil fertility. The...
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Enrichment of Naked Amoebae Species
In spite of various studies on naked amoebae species across the world, the diversity of naked amoebae in various habitats is still poorly studied....
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SEM: Sample Preparation, Fixation, and Staining of Protists
The ultrastructure and morphology of the protist can be studied using high-resolution electron microscope, i.e., scanning electron microscope (SEM)....
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Isolation and Cultivation and Staining of Paramecium
Paramecium is the microscopic, unicellular eukaryotic biological indicator organism explored for the study of freshwater ecotoxicity and various...
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Non-flooded Petri Plate Method to Study the Species Diversity of Flagellates
Flagellates have one to many flagella, which are used for motility, attachment to a substrate, and feeding. They differ phylogenetically. Non-flooded...
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Abundance Calculation of Naked and Testate Amoebae from Soil
Protists in soil perform important functions in the decomposer cycle and plant growth and are valued bioindicators for natural and anthropogenic...
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Abundance Calculation of Mycophagous Amoebae
Mycophagous protists occur in most soils, but their proper counting methods are lacking. As reported in the literature, mycophagous amoebae can be...
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Methodology to Study Species Diversity of Naked Amoeba and Testate Amoeba
The following method works well with the majority of soil and litter samples, even though most processes grossly underestimate the abundances of...
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Protargol Staining Technique for Identification of Soil Protists
Protargol staining technique is an excellent way to view the cellular organs of protists such as nuclei, cytostome, cilia, and flagella and to...
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Initial Observation of Protist from Soil
Soil is a complex and dynamic ecosystem that is home to a large number of microbial communities. These communities are composed of diverse groups of...
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Classical Wet Mount Method for Observing Live Protists
Protist ecology has used optical microscopes for observing cell features since very beginning. A dissecting microscope with light- and dark-field...
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Isolation and Enumeration of Mycophagous Protist
Protists can prey upon other smaller organisms such as bacteria and fungi. Their feeding actions are of utmost importance in driving the microbial...
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DNA Barcoding Techniques for Protists
The study of free-living protist populations in soil is gaining momentum due to their crucial role in soil ecosystems, particularly in carbon and...