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Enumeration of Testate Amoeba Through Direct Count from Soil
Several methods have been used to extract testate amoebae from various ecological niche to examine and quantify their tests using light microscopy.... -
Standard Solutions and Media Used for Isolation of Soil Protists
Protozoa are heterotrophic, nonfilamentous protists which play a major role in food web decomposition as bacterivores. In both terrestrial and... -
Soil Sampling, Processing, and Storage
Soil is a natural element that presents unique characteristics, and soil analysis has been the most used technique for diagnosing soil fertility. The... -
Axenic Cultivation of Soil Protists
Most of the physiological work on the protozoa has been restricted because the organism which need to be investigated cannot be raised in pure... -
SEM: Sample Preparation, Fixation, and Staining of Protists
The ultrastructure and morphology of the protist can be studied using high-resolution electron microscope, i.e., scanning electron microscope (SEM).... -
Most Probable Number (MPN) Method for Enumeration of Soil Protists
The most probable number (MPN) technique has been widely used to enumerate environmental microorganisms, including soil protists. This... -
DNA Extraction from Trophozoites and Cysts Using Manual Protocol
Protist taxa are ubiquitous members among soil microbial communities. Soil protistan communities are very diverse and yet to be explored due to the... -
Media Used for Enrichment of Soil Protists
The enrichment of protists highly depends upon the chemical composition of the synthetic medium next to the temperature. It affects growth and... -
Enrichment of Naked Amoebae Species
In spite of various studies on naked amoebae species across the world, the diversity of naked amoebae in various habitats is still poorly studied.... -
TEM: Sample Preparation, Fixation, and Staining of Protists
Transmission electron microscopy (TEM) is an ideal device to study the internal structure of cells and different types of biological materials, as... -
Classical Wet Mount Method for Observing Live Protists
Protist ecology has used optical microscopes for observing cell features since very beginning. A dissecting microscope with light- and dark-field... -
Abundance Calculation of Naked and Testate Amoebae from Soil
Protists in soil perform important functions in the decomposer cycle and plant growth and are valued bioindicators for natural and anthropogenic... -
Non-flooded Petri Plate Method to Study the Species Diversity of Flagellates
Flagellates have one to many flagella, which are used for motility, attachment to a substrate, and feeding. They differ phylogenetically. Non-flooded... -
Methodology to Study Species Diversity of Naked Amoeba and Testate Amoeba
The following method works well with the majority of soil and litter samples, even though most processes grossly underestimate the abundances of... -
Isolation and Cultivation and Staining of Paramecium
Paramecium is the microscopic, unicellular eukaryotic biological indicator organism explored for the study of freshwater ecotoxicity and various... -
Species Identification Through Sequencing
Ciliates with nuclear dualism are reported to be a highly divergent unicellular eukaryotes. They play very vital roles in regulation of microbial... -
Abundance Calculation of Mycophagous Amoebae
Mycophagous protists occur in most soils, but their proper counting methods are lacking. As reported in the literature, mycophagous amoebae can be... -
Isolation and Enumeration of Mycophagous Protist
Protists can prey upon other smaller organisms such as bacteria and fungi. Their feeding actions are of utmost importance in driving the microbial... -
Enrichment of Mycophagous Protists
Soil protists are considered to be bacterivorous and fungivorous where bacterial energy channel in food web is controlled by bacterivorous nematodes... -
Initial Observation of Protist from Soil
Soil is a complex and dynamic ecosystem that is home to a large number of microbial communities. These communities are composed of diverse groups of...