1,560 Result(s)
within
Protocol
Zi**g Wang
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Protocol
Organoid Culture of Different Intestinal Segments from Human and Mouse
The intestine comprises distinct segments, each characterized by unique cell populations and functions. Intestinal organoids faithfully replicate the cellular composition and functions of the intestine. Over t...
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Production of Neuroepithelial Organoids from Human-Induced Pluripotent Stem Cells for Mimicking Early Neural Tube Development
Organoids have emerged as robust tools for unravelling the mechanisms that underly tissue development. They also serve as important in vitro systems for studying fundamentals of stem cell behavior and for buil...
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Rapid Purification of dsRNA Using Micro-Spin Cellulose Column
Double-stranded RNA (dsRNA) is the replication intermediates of all RNA viruses. Purification and analysis of the profile and sequence of dsRNA is vital in virus diagnoses and/or characterization. Cellulose is...
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Workflow for Performing Genetic Manipulation in Human Trophoblast Stem Cells Using CRISPR/Cas9 Technology
Clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9-based tool kits greatly facilitate the investigation of gene function, especially gene editing and transcriptional regulation technology....
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Single-Cell Characterization of the Tumor Ecosystem in Liver Cancer
Tumor heterogeneity along with the complex landscape of the tumor microenvironment create critical challenges for effective liver cancer interventions. Characterizing the tumor ecosystem at the single-cell lev...
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Formation and Analysis of Biofilms in Vivo
Establishing a biofilm infection model in vivo allows a better understanding of the underlying infection mechanisms of bacteria. Here we describe a method for constructing an in vivo biofilm model of Streptococcu...
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Generation of Human Trophoblast Stem Cell-Dependent Placental In Vitro Models
Currently, human trophoblast stem cell (hTSC) is considered to be the most promising laboratory model stimulating trophoblast criteria. Our group has established hTSCs allowing differentiation to syncytiotroph...
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Metabolic Analysis of Intracellular Pathogenic Bacteria Using NMR
Pathogen proliferation and virulence depend on available nutrients, and these vary when the pathogen moves from outside of the host cell (extracellular) to the inside of the host cell (intracellular). Nuclear ...
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Directed Differentiation of Neurons from Human iPSCs for Modeling Neurological Disorders
Human-induced pluripotent stem cell (hiPSC) technology has enabled comprehensive human cell-based disease modeling in vitro. Due to limited accessibility of primary human neurons as well as species-specific di...
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Pop-Out Myocardial Infarction Induction in Mice
Myocardial infarction (MI) in mice is a widely used surgical model in preclinical cardiac repair studies to recapitulate human cardiovascular ischemic disease. Induction of reproducible infarct size is crucial...
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Profiling Human Cerebrospinal Fluid (CSF) Endogenous Peptidome in Alzheimer’s Disease
Human cerebrospinal fluid (CSF) is a rich source for central nervous (CNS)-related disease biomarker discovery due to its direct interchange with the extracellular fluid of the CNS. Though extensive proteome...
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Extraction of Proteins from Soil
Soil metaproteomics could explore the proteins involved in life activities and their abundance in the soils to overcome the difficulty in pure cultures of soil microorganisms and the limitations of proteomics ...
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Induction of Meiotic Initiation in Long-Term Mouse Spermatogonial Stem Cells Under Retinoid Acid and Nutrient Restriction Conditions
Spermatogonial stem cells (SSCs) produce haploid sperm via mitosis and meiosis in vivo. Although the technique to culture mouse SSCs has been well established, induction of meiosis in vitro has remained a chal...
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Automated Real-Time Monitoring of Extracellular pH to Assess Early Plant Defense Signaling
Extracellular alkalinization mediated by the inhibition of plasma membrane-located proton pum** ATPases hallmarks the initiation of defense signaling in plant cells. Early defense responses also include depo...
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Application of dsRNA in the Pine Wood Nematode, Bursaphelenchus xylophilus
The pine wood nematode Bursaphelenchus xylophilus is one of the most destructive invasive species worldwide, causing the wilting and eventual death of pine trees. Despite recognition of their economic and environ...
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Reconstructing Signaling Networks Using Biosensor Barcoding
Understanding how signaling networks are regulated offers valuable insights into how cells and organisms react to internal and external stimuli and is crucial for develo** novel strategies to treat diseases....
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Single-Cell Proteomics by Barcoded Phage-Displayed Screening via an Integrated Microfluidic Chip
Recent advancements in the profiling of proteomes at the single-cell level necessitate the development of quantitative and versatile platforms, particularly for analyzing rare cells like circulating tumor cell...
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Using Artificial Intelligence to Interpret Clinical Flow Cytometry Datasets for Automated Disease Diagnosis and/or Monitoring
Flow cytometry (FC) is routinely used for hematological disease diagnosis and monitoring. Advancement in this technology allows us to measure an increasing number of markers simultaneously, generating complex ...
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A Robust In Vitro Culture Model and Generation of Memory Monocytes
Innate monocytes can be trained or reprogrammed to adopt distinct memory states, such as low-grade inflammation and immune exhaustion, bearing fundamental relevance to the pathogenesis of both acute diseases s...
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Monitoring the Intracellular Trafficking of Virus-Induced Structures and Intercellular Spread of Viral Infection in Plants Using Endomembrane Trafficking Pathway-Specific Chemical Inhibitor and Organelle-Selective Fluorescence Dye
Infection by positive-strand RNA viruses induces extensive remodeling of the host endomembrane system in favor of viral replication and movement. The integral membrane protein 6K2 of potyviruses induces the fo...
