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Protocol
Absolute Quantification of TGF-β Signaling Proteins Using Quantitative Western Blot
Cell signaling governs the basic functions of cells by molecular interactions that involve of many proteins. The abundance of signaling proteins can directly influence cellular responses to external signal, co...
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Protocol
Optogenetic Control of TGF-β Signaling
Cells employ signaling pathways to make decisions in response to changes in their immediate environment. The Transforming Growth Factor β (TGF-β) signaling pathway plays pivotal roles in regulating many cellul...
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Protocol
Measuring TGF-β Ligand Dynamics in Culture Medium
TGF-β plays an important role in a myriad of cell activities including differentiation, proliferation, and growth arrest. These effects are influenced by the concentration of TGF-β in the surrounding milieu, w...
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Protocol
A Tutorial on Mathematical Modeling of Biological Signaling Pathways
Mathematical models have been widely used in the studies of biological signaling pathways. Among these studies, two systems biology approaches have been applied: top-down and bottom-up systems biology. The for...
