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Protocol
Assays for the Spectrum of Circulating Tumor Cells
Cancer cells sharing stem cell properties are called “cancer stem cells” (CSCs). CSCs have distinct metabolic properties, are intrinsically drug resistant evading chemotherapies, are regulated by miRNA network...
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Protocol
Virus-Induced Gene Silencing in Rose Flowers
Virus-induced gene silencing (VIGS) is a favorable method to study gene function by posttranscriptional gene silencing in plants. Here we describe a methodology of graft-accelerated VIGS in rose aimed at obtai...
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Protocol
Virus-Induced Gene Silencing and Agrobacterium tumefaciens-Mediated Transient Expression in Nicotiana tabacum
Virus-induced gene silencing (VIGS) is a rapid method for transient silencing of plant genes. In this chapter, we describe the methodology for Tobacco rattle virus (TRV)-based VIGS in Nicotiana tabacum. In combin...
