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    Protocol

    Production of Cell Surface and Secreted Glycoproteins in Mammalian Cells

    Mammalian protein expression systems are becoming increasingly popular for the production of eukaryotic secreted and cell surface proteins. Here we describe methods to produce recombinant proteins in adherent ...

    Elena Seiradake, Yuguang Zhao, Weixian Lu, A. Radu Aricescu… in Structural Proteomics (2015)

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    Protocol

    Mutagenesis of the Repeat Regions of Herpesviruses Cloned as Bacterial Artificial Chromosomes

    Cloning of infectious and pathogenic herpesvirus genomes in a bacterial artificial chromosome (BAC) vector greatly facilitates genetic manipulation of their genomes. BAC-based mutagenesis strategies of viruses...

    Yuguang Zhao, Venugopal Nair in In Vitro Mutagenesis Protocols (2010)