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    Protocol

    Isolation of Extracellular Vesicles for Proteomic Profiling

    Extracellular vesicles are nano-sized lipid bilayer vesicles released from most cells, including archaea, bacteria, and eukaryotic cells. These membrane vesicles play multiple roles in cell-to-cell communicati...

    Dong-Sic Choi, Yong Song Gho in Proteomic Profiling (2015)

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    Protocol

    Cloning-Independent Expression and Screening of Enzymes Using Cell-Free Protein Synthesis Systems

    We present a strategy for expression and screening of microbial enzymes without involving cloning procedures. Libraries of putative ω-transaminases (ω-TA) and mutated Candida antarctica lipase B (CalB) are PCR-am...

    Yong-Chan Kwon, Jae-Kwang Song, Dong-Myung Kim in Cell-Free Protein Synthesis (2014)