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Protocol
Whole Genome Sequencing of Porcine Epidemic Diarrhea Virus and Porcine Deltacoronavirus
Swine enteric coronaviruses, such as porcine epidemic diarrhea virus (PEDV) and porcine deltacoronavirus (PDCoV), are among the most important pathogens in the swine industry. The diseases due to infection of ...
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Protocol
Simultaneously Assessing Concentration Changes in 20 Biochemical Pathways as a Result of Drug Dosing and Cytochrome P450 and Non-cytochrome P450-Mediated Metabolism: An Untargeted Metabolomics GC/MS Assay
When a drug is dosed into a hepatic cell culture, an animal, or a human, dozens of intracellular metabolites that are structurally related to the drug molecule are produced from a number of cellular metabolism...
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Protocol
In Situ Hybridization and Immunostaining of Xenopus Brain
The dynamic expression pattern analysis provides the primary information of gene function. Differences of the RNA and/or protein location will provide valuable information for gene expression regulation. Gener...
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Protocol
Common Methods Used for the Discovery of Natural Anticancer Compounds
Carcinogenesis is a long-term, multifactorial, and multistep process. Dietary phytochemicals can play a significant role in cancer prevention. In this chapter, we describe common protocols to study the role of...