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Protocol
Imaging and Tracking Stem Cell Engraftment in Ischemic Hearts by Near-Infrared Fluorescent Protein (iRFP) Labeling
Stem cell-based therapies hold great promise as alternative therapeutic strategies for various chronic diseases, including ischemic cardiomyopathy. Tracking the engraftment of transplanted stem cells is critic...
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Protocol
Chiral Separation by NACE Using Polyol Derivative–Boric Acid Complexes
Nonaqueous capillary electrophoresis (NACE) is an effective method for chiral separation. Many polyol derivatives (e.g., d-(+)-xylose, lactobionic acid, diacetone-d-mannitol, l-sorbose, and d-gluconic acid δ-lact...
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Protocol
FRAP-Based Analysis of Rho GTPase-Dependent Polar Exocytosis in Pollen Tubes
The regulation of exocytosis by Rho GTPases is conserved in eukaryotic kingdoms. Given the spatiotemporal dynamics of Rho GTPase activity, a method for visualizing exocytosis with high space and time resolutio...
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Protocol
The LC-MS/MS Methods for the Determination of Specific Antibiotics Residues in Food Matrices
This chapter describes the LC-MS/MS methods for the determination of antibiotics residues in food matrices. The types of antibiotics include β-lactam antibiotics, sulfonamides, tetracyclines, fluoroquinolones,...
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Protocol
Polyelectrolyte Coatings for Microchip Capillary Electrophoresis
In chip-based electrophoretic analysis of biomolecules, chemical modification of the microchannel is widely employed to reduce or eliminate the analyte-wall interactions and alter electroosmotic flow (EOF) in ...