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    Protocol

    Microscale Solution Isoelectrofocusing

    Proteomics is a relatively new research discipline that deals with systematic, large-scale analyses of proteins in biological systems (1,2). Two-dimensional polyacrylamide gel electrophoresis (2-D PAGE) is curren...

    Xun Zuo, David W. Speicher in Protein Purification Protocols (2004)

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    Protocol

    Tissue-Specific Ribosome Profiling in Drosophila

    Robust mechanisms exist that serve to dynamically regulate the translation of mRNA into proteins across heterogeneous tissues. These processes ensure timely generation of proteins in quantities that scale with...

    Xun Chen, Dion Dickman in Ribosome Profiling (2021)