241 Result(s)
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Protocol
Targeted Modification of Epigenetic Marks Using CRISPR/dCas9-SunTag-Based Modular Epigenetic Toolkit
The epigenome, consisting of chemical modifications to DNA and histone proteins, can alter gene expression. Clustered regularly interspaced short palindromic repeats/dead CRISPR-associated protein 9 (CRISPR/dC...
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Protocol
Measuring Canopy Gas Exchange Using CAnopy Photosynthesis and Transpiration Systems (CAPTS)
Canopy photosynthesis (Ac), rather than leaf photosynthesis, is critical to gaining higher biomass production in the field because the daily or seasonal integrals of Ac correlate with the daily or seasonal integr...
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Protocol
Quantifying the Kinetics of Pilus-Specific Sortase-Catalyzed Crosslinking Using High-Performance Liquid Chromatography
Gram-positive bacteria display pili whose protein components (pilins) are covalently crosslinked by pilus-specific sortase enzymes. These cysteine transpeptidase enzymes catalyze a transpeptidation reaction th...
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Protocol
Construction of Xylose-Utilizing Cyanobacterial Chassis for Bioproduction Under Photomixotrophic Conditions
Xylose is a major component of lignocellulose and the second most abundant sugar present in nature after glucose; it, therefore, has been considered to be a promising renewable resource for the production of b...
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Protocol
An Overview of High-Throughput Crop Phenoty**: Platform, Image Analysis, Data Mining, and Data Management
In this chapter, we explore the application of high-throughput crop phenoty** facilities for phenotype data acquisition and the extraction of significant information from the collected data through image pro...
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Protocol
Generation of a Naïve Human scFv Phage Display Library and Panning Selection
Phage display antibody libraries have been successfully used as the essential tool to produce monoclonal against a plethora of targets ranging from diseases to native biologically important proteins as well ...
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Protocol
Co-staining with Fluorescent Antibodies and Antibody-Derived Tags for Cell Sorting Prior to CITE-Seq
The paired detection of the transcriptome and proteome at single-cell resolution provides exquisite insight to immune mechanisms in health and disease. Here, we describe a detailed protocol wherein we combine ...
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Protocol
Monitoring Autophagy with GFP-LC3 Reporter
Autophagy is a key process that maintains cellular homeostasis. Autophagy contributes to various physiological and pathophysiological processes. Development of methodologies for autophagy detection has greatly...
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Protocol
Identification of Plant Co-regulatory Modules Using CoReg
Regulatory network is often characterized by complex interactions between transcription factors (TFs) and target genes. The synergistic regulations among multiple TFs may co-induce/co-suppress the expressions ...
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Protocol
Modeling Plant Transcription Factor Networks Using ConSReg
Plants have developed complex regulatory programs to respond to various environmental stress such as heat, drought, and cold. Systematic understanding of these biological processes depends on robust constructi...
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Protocol
Surface Modification of Liposomes Using Folic Acid
Liposomes are usually defined as spherically shaped microscopic vesicles that consist of one or more phospholipid bilayer membranes. They are widely used in drug delivery due to their biocompatibility, biodegr...
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Protocol
A Clickable NAD+ Analog-Based Assay of Poly(ADP-Ribosyl)ated Proteins
Poly(ADP-ribosyl)lation (PARylation) is a posttranslational modification that plays an important role in a variety of biological processes in both animals and plants. Identification of PARylated substrates is ...
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Protocol
Isolation of Hepatic Stellate Cells and Lymphocytes for Co-culture Systems
In contrast to quiescent hepatic stellate cells (HSCs), activated HSCs play crucial roles in the development of liver fibrosis by producing a huge amount of extracellular matrix such as collagen fibers. Howeve...
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Protocol
Pseudouridine Identification and Functional Annotation with PIANO
Pseudouridine (Ψ) is the first-discovered RNA modification abundantly present in many classes of RNAs, which plays a pivotal role in a series of biological processes. Accurately identifying the location of Ψ s...
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Protocol
Generation of Rabbit Chimeras by Eight-Cell Stage Embryo Injection
Pluripotent stem cell (PSC) injection to the blastocyst stage embryos is a widely used method to evaluate the pluripotency through chimeric contribution. It is routinely used to produce transgenic mice. Howeve...
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Protocol
The Delivery of ABE mRNA to the Adult Murine Liver by Lipid Nanoparticles (LNPs)
A genetic disorder is a disease caused by an abnormal DNA sequence, and almost half of the known pathogenic monogenetic mutations are caused by G-to-A mutation (Landrum et al., Nucleic Acids Res 44:D862–868, 2...
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Protocol
Delineating the Role of Toll-Like Receptors in Inflammatory Bowel Disease
recognize altered gut microbiota triggering an immune response. These responses play a critical role in the pathogenesis and treatment of inflammatory bowel disease (IBD). IBD is characterized by inflammation...
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Protocol
Method to Generate Dorsal Forebrain Brain Organoids from Human Pluripotent Stem Cells
Region-specific brain organoids, such as dorsal forebrain brain organoid, have become increasingly useful to model early brain development. Importantly, these organoids provide an avenue to investigate mechani...
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Protocol
Introduction and Perspectives of DNA Base Editors
DNA base editors, one of the CRISPR-based genome editing tools, can induce targeted point mutations at desired sites. Their superiority is based on the fact that they can perform efficient and precise gene edi...
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Protocol
Targeted Mutagenesis of Mycobacterium Strains by Homologous Recombination
Targeted mutagenesis by homologous recombination (TMHR) is an efficient allelic exchange mutagenesis for bacterial genome engineering in synthetic biology. Unlike other allelic exchange methods, TMHR does not ...
