16 Result(s)
within
Protocol
Wen-**g Zhang
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Protocol
GFP-LC3 High-Content Assay for Screening Autophagy Modulators
Autophagy plays an important role in maintaining cellular homeostasis. Defects in autophagy have been linked to various human diseases, such as cancer, neurodegenerative diseases, and cardiovascular diseases. ...
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Protocol
Comparative Analysis of Core and Accessory Genes in Coexpression Network
Prokaryotes harbor a various proportion of accessory genes in their genomes. The integration of accessory functions with the core regulation network is critical for environmental adaptation, particularly consi...
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Protocol
Metagenomic Assembly: Reconstructing Genomes from Metagenomes
Assembly of metagenomic sequence data into microbial genomes is of critical importance for disentangling community complexity and unraveling the functional capacity of microorganisms. The rapid development of ...
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Protocol
Episomal Reprogramming of Human Peripheral Blood Mononuclear Cells into Pluripotency
Peripheral blood is an easily accessible cell resource for reprogramming into pluripotency by episomal vectors. Here, we describe an approach for efficient generation of integration-free induced pluripotent st...
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Protocol
Engineering T Cells Using CRISPR/Cas9 for Cancer Therapy
Recent advances in the development of gene editing technologies, especially the CRISPR/Cas 9 system, have substantially enhanced our ability to make precise and efficient changes in the genomes of various cell...
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Protocol
Application of CRISPR/Cas9-Mediated Genome Editing in Leishmania
CRISPR-Cas9 is an RNA guided endonuclease derived from the bacterium Streptococcus pyogenes. Due to its simplicity, versatility, and high efficiency, it has been widely used for genome editing in a variety of org...
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Protocol
SpyTag–SpyCatcher Chemistry for Protein Bioconjugation In Vitro and Protein Topology Engineering In Vivo
The emergence of “molecular superglue,” such as SpyTag–SpyCatcher chemistry, has tremendously expanded our capability in manipulating protein shape and architecture via conjugation. Telechelic proteins bearing...
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Protocol
Chimeric Mouse Generation by ES Cell Blastocyst Microinjection and Uterine Transfer
The ability to generate chimeric mice through microinjecting embryonic stem (ES) cells into blastocysts is a critical step for the conventional ES cell-mediated knockout technology. In recent years, designer n...
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Protocol
Using CRISPR/Cas9 for Gene Knockout in Immunodeficient NSG Mice
NOD.Cg-Prkdcscid Il2rgtm1Wjl/SzJ (NSG) mice are an immunodeficient strain that enables human cell xenografts. However, NSG mice possess a complex genetic background that would complicate cross-breeding with other...
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Protocol
Application of TALE-Based Approach for Dissecting Functional MicroRNA-302/367 in Cellular Reprogramming
MicroRNAs are small 18–24 nt single-stranded noncoding RNA molecules involved in many biological processes, including stemness maintenance and cellular reprogramming. Current methods used in loss-of-function s...
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Protocol
QuickRNASeq: Guide for Pipeline Implementation and for Interactive Results Visualization
Sequencing of transcribed RNA molecules (RNA-Seq) has been used wildly for studying cell transcriptomes in bulk or at the single-cell level (Wang et al., Nat Rev Genet, 10:57–63, 2009; Ozsolak and Milos, Nat R...
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Protocol
Capture Hybridization of Long-Range DNA Fragments for High-Throughput Sequencing
Capture hybridization coupled with high-throughput sequencing (HTS) has become one of the most popular approaches to address some scientific problems not only for fundamental evolution but also for ecology and...
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Protocol
Screening Leishmania donovani Complex-Specific Genes Required for Visceral Disease
Leishmania protozoan parasites are the causing agent of leishmaniasis. Depending on the infecting species, Leishmania infection can causes a wide variety of diseases such as self-healing cutaneous ...
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Protocol
Prediction of Conformational B-Cell Epitopes
Conformational B-cell epitopes play an important role in the epitope-based vaccine design. The increase of available data promotes the development of computational methods. Compared with the wet experiments, t...
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Protocol
Genome-Wide Identification of MicroRNAs in Medicago truncatula by High-Throughput Sequencing
MicroRNAs (miRNAs) are small, endogenous RNAs that play important regulatory roles in development and stress response in plants by negatively regulating gene expression post-transcriptionally. Medicago truncatula
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Protocol
Use of Rolling-Circle Amplification for Large-Scale Yeast Two-Hybrid Analyses
Detection of protein–protein interactions on a large-scale has become a major focus of functional genomics after the completion of genome sequencing. The information generated from these studies not only assem...
