6 Result(s)
within
Life Sciences
Biological Techniques
Sung-** Kim
Protocol
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Protocol
Near-Infrared Bioluminescence Imaging of Animal Cells with Through-Bond Energy Transfer Cassette
is the most general substrate for marine . The present protocol introduces a near-infrared (NIR ) bioluminescence (BL) of mammalian cells with a cyanine-5 (Cy5) dye-conjugated CTZ . This unique Cy5-conjuga...
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Protocol
Highly Bright NIR-BRET System for Imaging Molecular Events in Live Cells
The present protocol demonstrates a novel mammalian cell imaging platform exerting a bioluminescence resonance energy transfer (BRET) system. This platform achieves a ~300 nm blue-to-near infrared shift of the...
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Protocol
Ligand-Activatable BRET9 Probes for Imaging Molecular Events in Living Mammalian Cells
Bioluminescence resonance energy transfer (BRET) is a commonly used assay system for studying protein-protein interactions. The present protocol introduces a conceptually unique ligand-activatable BRET system ...
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Protocol
A New Lineage of Artificial Luciferases for Mammalian Cell Imaging
The present protocol introduces a new lineage of artificial luciferases (ALucs) with unique optical properties for mammalian cell imaging. The primary candidate sequence was first created with a sequence logo ...
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Protocol
Azide- and Dye-Conjugated Coelenterazine Analogues for Imaging Mammalian Cells
Coelenterazine (CTZ) is a common substrate to most marine luciferases and photoproteins. The present protocol introduces mammalian cell imaging with nine novel dye- and azide-conjugated CTZ analogues, which we...
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Protocol
Luciferase-Specific Coelenterazine Analogues for Optical Cross Talk-Free Bioassays
Spectral overlaps in fluorescence (FL) and bioluminescence (BL) commonly cause optical cross talks. The present protocol introduces five different lineages of coelenterazine (CTZ) analogues, which have selecti...
