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  1. Protocol

    Detection of RNA Modifications by HPLC Analysis and Competitive ELISA

    Over 100 different RNA modifications exist that are introduced posttranscriptionally by enzymes at specific nucleotide positions. Ribosomal RNA (rRNA) and transfer RNA (tRNA) exhibit the most and diverse modif...

    Gernot Nees, Andreas Kaufmann, Stefan Bauer in Innate DNA and RNA Recognition (2014)

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    Protocol

    Cloning and Expression of Hemicellulases from Aspergillus nidulans in Pichia pastoris

    The methylotrophic yeast Pichia pastoris is increasingly used for heterologous expression of high quality proteins in laboratory-scale (milligram) quantities. Commercially available polysaccharide-active enzyme p...

    Prasanna Vasu, Stefan Bauer, Brett J. Savary in Recombinant Gene Expression (2012)