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Protocol
Detection of RNA Modifications by HPLC Analysis and Competitive ELISA
Over 100 different RNA modifications exist that are introduced posttranscriptionally by enzymes at specific nucleotide positions. Ribosomal RNA (rRNA) and transfer RNA (tRNA) exhibit the most and diverse modif...
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Protocol
Cloning and Expression of Hemicellulases from Aspergillus nidulans in Pichia pastoris
The methylotrophic yeast Pichia pastoris is increasingly used for heterologous expression of high quality proteins in laboratory-scale (milligram) quantities. Commercially available polysaccharide-active enzyme p...