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Protocol
Efficiently Editing Multiple Duplicated Homeologs and Alleles for Recurrent Polyploids
Research on the evolutionary fate of duplicated genes in recurrent polyploids is scarce due to the difficulties in disentangling the different homeologs and alleles of duplicated genes. This chapter describes ...
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Protocol
Accurate Proteoform Identification and Quantitation Using pTop 2.0
The remarkable advancement of top-down proteomics in the past decade is driven by the technological development in separation, mass spectrometry (MS) instrumentation, novel fragmentation, and bioinformatics. H...
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Protocol
MeDIP-on-Chip for Methylation Profiling
DNA methylation is an important regulatory step in gene expression. Knowledge of the alterations in DNA methylation at a whole genome scale improves our understanding of gene regulation and potential correlati...
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Protocol
Animal Models of Inflammatory Pain
Animal models of inflammatory pain have been widely used to study the mechanisms of tissue injury-induced persistent pain. A variety of inflammatory agents or irritants, including complete Freund’s adjuvant, c...