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    Protocol

    Efficiently Editing Multiple Duplicated Homeologs and Alleles for Recurrent Polyploids

    Research on the evolutionary fate of duplicated genes in recurrent polyploids is scarce due to the difficulties in disentangling the different homeologs and alleles of duplicated genes. This chapter describes ...

    Rui-Hai Gan, Li Zhou, Jian-Fang Gui in Polyploidy (2023)

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    Protocol

    Accurate Proteoform Identification and Quantitation Using pTop 2.0

    The remarkable advancement of top-down proteomics in the past decade is driven by the technological development in separation, mass spectrometry (MS) instrumentation, novel fragmentation, and bioinformatics. H...

    Rui-**ang Sun, Rui-Min Wang, Lan Luo, Chao Liu, Hao Chi in Proteoform Identification (2022)

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    Protocol

    MeDIP-on-Chip for Methylation Profiling

    DNA methylation is an important regulatory step in gene expression. Knowledge of the alterations in DNA methylation at a whole genome scale improves our understanding of gene regulation and potential correlati...

    Yaw-Wen Hsu, Rui-Lan Huang, Hung-Cheng Lai M.D., Ph.D. in Cervical Cancer (2015)

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    Protocol

    Animal Models of Inflammatory Pain

    Animal models of inflammatory pain have been widely used to study the mechanisms of tissue injury-induced persistent pain. A variety of inflammatory agents or irritants, including complete Freund’s adjuvant, c...

    Rui-**n Zhang, Ke Ren in Animal Models of Pain (2011)