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    Protocol

    Design, Construction, and Validation of Artificial MicroRNA Vectors Using Agrobacterium-Mediated Transient Expression System

    Artificial microRNA (amiRNA) technology utilizes microRNA (miRNA) biogenesis pathway to produce artificially selected small RNAs using miRNA gene backbone. It provides a feasible strategy for inducing loss of ...

    Basdeo Bhagwat, Ming Chi, Dianwei Han… in Biotechnology of Plant Secondary Metabolism (2016)

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    Protocol

    Knockdown of Polyphenol Oxidase Gene Expression in Potato (Solanum tuberosum L.) with Artificial MicroRNAs

    It is of great importance and interest to develop crop varieties with low polyphenol oxidase (PPO) activity for the food industry because PPO-mediated oxidative browning is a main cause of post-harvest deterio...

    Ming Chi, Basdeo Bhagwat, Guiliang Tang… in Biotechnology of Plant Secondary Metabolism (2016)