6 Result(s)
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Protocol
Virus Isolation and Preparation of Sucrose-Banded Chikungunya Virus Samples for Transmission Electron Microscopy
Virus isolation and purification is an invaluable technique in virology to detect and characterize viruses. This chapter describes a large-scale Chikungunya virus (CHIKV) propagation and purification methods b...
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Protocol
Atomic Force Microscopy of Plasmodium-Infected Red Blood Cells: Detecting and Localizing Single Molecular Recognition Events
Atomic Force Microscopy (AFM) is a powerful tool for exploring the interaction between ligands and receptors, as well as their exact locations on the red cell surface. Here we discuss current and future applic...
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Protocol
Matrix Metalloproteinase Expression in Lung Cancer
The importance of matrix metalloproteinases (MMPs) in the growth and spread of solid tumors has been known for over a decade (1,2). However, the molecular mechanisms that regulate their expression and the elucida...
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Protocol
Current In Vitro Models of Leukocyte Migration
A large component of airway inflammatory disease is the recruitment of activated leukocytes (primarily eosinophils and T lymphocytes) from the lung vasculature into the bronchial walls resulting in lung edema....
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Protocol
Expression of IL-10 and GM-CSF in Blood Monocytes and Alveolar Macrophages
Peripheral blood monocytes (PBMs) and alveolar macrophages (AMs) are widely recognized as cells that play a central role in the regulation of immune and inflammatory activities, as well as in tissue remodeling...
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Protocol
Use of the Polymerase Chain Reaction Technique to Detect the t(14;18) Translocation in Lymphoid Tissue
The t(14;18) (q32;21) chromosomal translocation is characteristic of follicle center cell lymphomas, involving 95% of cases (1,2). In addition, it has been found in a variety of other malignant lymphomas, includi...
