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    Protocol

    Jasmonic Acid–Amino Acid Conjugation Enzyme Assays

    Jasmonic acid (JA) is activated for signaling by its conjugation to isoleucine (Ile) through an amide linkage. The Arabidopsis thaliana JASMONIC ACID RESISTANT1 (JAR1) enzyme carries out this Mg-ATP-dependent rea...

    Martha L. Rowe, Paul E. Staswick in Jasmonate Signaling (2013)